Generating Schwann Cell-Like Cells from Bone Marrow Stromal Cells

In This Article

Abstract

Source: Tsui, Y., et al. Hypoxic Preconditioning of Marrow-derived Progenitor Cells As a Source for the Generation of Mature Schwann Cells. J. Vis. Exp. (2017)

This video demonstrates a method for obtaining Schwann cell-like cells from multipotent bone marrow stromal cells (BMSCs). Initially, the BMSCs undergo hypoxia treatment, followed by directed differentiation into neuronal progenitor cells. Subsequently, specific media and culture conditions are employed to induce the transformation of these progenitor cells into Schwann cell-like cells.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Preparation of Rat MSC Cultures

Harvest of MSCs from the femur
1. Autoclave all dissection tools (i.e., fine dissecting scissors, blunt-tipped cutting scissors, and toothed forceps) at 180 °C for at least 2 h before use.
2. Prepare MSC growth medium comprising of minimal essential medium - alpha modification (αMEM) supplemented with 15% fetal bovine serum (FBS) and penicillin/streptomycin (P/S, 1% v/v).
3. Sacrifice young male Sprague Da....

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Results

Cell culture images A-D under microscope at varied magnifications, showing cell morphology and density.
Figure 1: Establishment of MSC colonies. Sizeable MSC colonies should be visible 6-7 days after the plating of bone marrow cells onto tissue culture plastic. A representative image of a rat MSC colony is shown (A), while human coloni.......