Handling Neuronal Stem Cell Cultures in a Cell Production Facility

In This Article

Abstract

Source: Stover, A. E., et al,. Culturing human pluripotent and neural stem cells in an enclosed cell culture system for basic and preclinical research. J. Vis. Exp. (2016).

The video outlines the procedure for maintaining a controlled environment in a cell culture workstation for the growth and maintenance of neuronal stem cells. These procedures involve setting chamber conditions, cleaning protocols, and media preparation, Thus, maintaining maximum sterility and reproducibility and replacing the traditional methods.

Protocol

1. Initial Setup

Setting Gas and Temperature Concentrations
1. Using the software, click the "Guest" tab on the upper left corner of the graphical interface. Log in to the system using a designated username and password. Make sure each user has their username and password.
2. Click on a module to adjust (Figure 1). Within the new window displaying the current settings, click on the existing O₂ set point value below "Set point" and enter this module's required O₂ concentration level. Enter 5% O₂ if pluripotent stem cells will be grown or manipulated in this module and 9% O

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Results

Process control interface showing CO2 and O2 levels, setpoints, chamber pressure, with control options.
Figure 1. Graphical Interface for the Cell Production Facility. (A) The graphical representation of the CPF is the default screen and matches the CAD drawing shown in Figure 3. A mouse click over Buffer Module 1 opens its control scr.......