Generation of Medial Thalamus-Anterior Cingulate Cortex Slices from Mouse Brains

Abstract

Source: Lu, H., et al. Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation. J. Vis. Exp. (2016)

The video demonstrates the preparation of medial thalamus-anterior cingulate cortex (MT-ACC) slices from mouse brains. A mouse's brain is dissected, cuts are made to expose the MT-ACC pathway, and slices are generated using a vibratome. The MT-ACC slices are then maintained in oxygenated artificial cerebrospinal fluid.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Brain Slice Preparation

1. Use male C57BL/6J mice, 4-8 weeks old. House the animals in an air-conditioned room (21-23 °C; 50% humidity; 12 hr/12 hr light /dark cycle, lights on at 8:00 AM) with free access to food and water.
2. Take a 250 ml aliquot of the artificial cerebrospinal fluid (aCSF) and place it in a beaker that contains ice. At the same time, supply continuous gas that is composed of 95% O₂ and 5% CO₂.
3. Su....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Anesthetic:
Isoflurane	Halocarbon Products Corporation	NDC 12164-002-25	4%
aCSF (total:1L):
D(+)-Glucose	MERCK	1.08337.1000	10 mM
Sodium hydrogen carbonate	MERCK	1.06329.0500	25 mM
Sodium chloride	MERCK	1.06404.1000	124 mM
(+)-Sodium L-ascorbate, >=98%	SIGMA	A4034-100G	0.15 g / 2 c.c
Magnesium sulfate, anhydrous, ReagentPlus	SIGMA	M7506-500G	2 mM
Calcium chloride dihydrate	MERCK	1.02382.1000	2 mM
Sodium dihydrogen phosphate monohydrate	MERCK	1.06346.1000	1 mM
Potassium chloride	May & Baker LTD Dagenham England	MS 7616	4.4 mM