1. Light Exposure Conditions
<ol>
	<li>From the larva stage until eclosion, keep the flies in a 12 h light/12 h dark cycle (LD) at 25 &#176;C (Figure 1A).</li>
	<li>Collect the flies 0 - 6 h after eclosion and place them into a new vial containing fly food.</li>
	<li>Set the vials into a transparent acrylic resin rack with three steps (a height of 41 cm, a base of 21 cm, a thickness of 4 cm, and a height of 13 cm for each step) (Figure 1B).</li>
	<li>Adjust the distance between the rack and an LED panel to provide illumination with an average intensity of 1,000 lux using a digital light meter (Figure 1C).</li>
	<li>Keep the flies for 1 - 3 d under one of the following conditions: constant darkness (DD), 12 h light/12 h dark cycle (LD), or constant light (LL) at 25 &#176;C in a small incubator (Figures 1A and 1C).</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Vial</td>
			<td>Hightech, Japan</td>
			<td>MKC-20</td>
			<td></td>
		</tr>
		<tr>
			<td>Plug</td>
			<td>Thermo Fisher Sciehtific, USA</td>
			<td>AS-275</td>
			<td></td>
		</tr>
		<tr>
			<td>Customized transparent rack made of acrylic resin</td>
			<td>Shin-Shin Corporation, Japan</td>
			<td></td>
			<td>a height of 41 cm, a base of 21 cm, a thickness of 4 cm and a height of 13 cm for each step</td>
		</tr>
		<tr>
			<td>Cool incubator</td>
			<td>MITSUBISHI ELECTRIC, Japan</td>
			<td>CN-40A</td>
			<td></td>
		</tr>
		<tr>
			<td>LED panel</td>
			<td>MISUMI, Japan</td>
			<td>LEDXC170-W</td>
			<td></td>
		</tr>
		<tr>
			<td>Digital light meter</td>
			<td>CEM</td>
			<td>DT-1301</td>
			<td></td>
		</tr>
		<tr>
			<td>Fly pad</td>
			<td>Tokken, Japan</td>
			<td>TK-HA03-S</td>
			<td></td>
		</tr>
	</tbody>
</table>

synaptic modulation in drosophila after exposure to prolonged light

Synaptic Modulation in Drosophila After Exposure to Prolonged Light

Source: Sugie, A., et al. <a href='https://app.jove.com/v/55176'>Analyzing Synaptic Modulation of Drosophila melanogaster Photoreceptors after Exposure to Prolonged Light.</a> J. Vis. Exp. (120)
This video demonstrates the rearing of Drosophila melanogaster under specific light conditions to study the role of Bruchpilot proteins in neurotransmitter release and the resulting modulation of synaptic activity.

<img alt="Chronobiology experiment setup: photoperiod diagram, test tube storage, light/flies chamber." src="/files/ftp_upload/22855/22855_Figure1.jpg" data-altupdatedat="1753371239000" data-alt="Figure 1" /> 
Figure 1: Preparation of Conditions for Exposure to Defined Light Intensity. (A) Light exposure protocols after eclosion. DD, continuous darkness; LD, 12 h light/12 h dark; LL, constant exposure to light. Fly brains were dissected at the times indicated in the ...

1. Light Exposure Conditions

	From the larva stage until eclosion, keep the flies in a 12 h light/12 h dark cycle (LD) at 25 &#176;C (Figure 1A).
	...

Begin with freshly eclosed Drosophila melanogaster flies in a collection vial containing nutrients.
Place these vials in a transparent acrylic rack.
Position the rack inside an incubator equipped with an internal light source at a precise distance to provide uniform and desired light exposure.
Rear the flies under these light conditions for a prolonged period.
In Drosophila eyes, the photoreceptor cells act as sensory neurons, detecting the light and converting it into electrical signals.
These signals travel along the axon and reach the synaptic terminals, where they connect with other neurons.
These terminals possess the Bruchpilot proteins, which facilitate the fusion of neurotransmitter-carrying vesicles to the cell membrane, allowing neurotransmitter release.
The released neurotransmitters bind to the receptors on the postsynaptic neurons to transmit the electrical signal, leading to synaptic activity.
 With continuous exposure to light, the Bruchpilot protein level decreases, reducing neurotransmitter release and resulting in the plasticity or modulation of synaptic activity. 

For this experiment, collect the flies into normal vials within six hours of occlusion. Load the collection vials into a transparent acrylic rack. In a small incubator, set to 25 degrees Celsius, position the rack at a precise distance from an LED panel where the light exposure is at an average of 1,000 lux. Then, rear flies for one to three days, using one of the following conditions, either constant darkness, 12 hours of light followed by 12 hours of darkness, or constant light.

synaptic-modulation-in-drosophila-after-exposure-to-prolonged-light

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715 Views. Begin with freshly eclosed Drosophila melanogaster flies in a collection vial containing nutrients. Place these vials in a transparent acrylic rack. Position the rack inside an incubator equipped with an internal light source at a precise distance to provide uniform and desired light exposure. Rear the flies under these light conditions for a prolonged period. In Drosophila eyes, the photoreceptor cells act as sensory neurons, detecting the light ...

Synaptic Modulation in Drosophila After Exposure to Prolonged Light

Abstract

Protocol

<img title="figure-results-1" alt="Chronobiology experiment setup: photoperiod diagram, test tube storage, light/flies chamber." src="/files/ftp_upload/22855/22855_Figure1.jpg" data-altupdatedat="1753371239000" data-alt="Figure 1" /> 
Figure 1: Preparation of Conditions for Exposure to Defined Light Intensity. (A) Light exposure protocols after eclosion. DD, continuous darkness; LD, 12 h light/12 h dark; LL, constant exposure to light. Fly brains were dissected at the times indicated in the ...

Synaptic Modulation in Drosophila After Exposure to Prolonged Light

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Name	Company	Catalog Number	Comments
Vial	Hightech, Japan	MKC-20
Plug	Thermo Fisher Sciehtific, USA	AS-275
Customized transparent rack made of acrylic resin	Shin-Shin Corporation, Japan		a height of 41 cm, a base of 21 cm, a thickness of 4 cm and a height of 13 cm for each step
Cool incubator	MITSUBISHI ELECTRIC, Japan	CN-40A
LED panel	MISUMI, Japan	LEDXC170-W
Digital light meter	CEM	DT-1301
Fly pad	Tokken, Japan	TK-HA03-S