Simultaneous Eye Tracking and Single-Neuron Recording to Identify Target-Selective Neurons

In This Article

Abstract

Source: Wang, S., et al. Simultaneous Eye Tracking and Single-Neuron Recordings in Human Epilepsy Patients. J. Vis. Exp. (2019).

The video demonstrates an assay to identify target-selective neurons via simultaneous eye-tracking and single-neuron recordings. A human participant is tasked with identifying a target object among an array of objects in a display, and the eye movement is tracked. Simultaneously, single neurons' firing rate is recorded via electrodes implanted in the brain. The data from both recordings are correlated to assess the presence of target-selective neurons.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board.

1. Participants

Recruit neurosurgical patients with intractable epilepsy who are undergoing placement of intracranial electrodes to localize their epileptic seizures.
Insert depth electrodes with embedded microwires into all clinically indicated target locations, which typically include a subset of amygdala, hippocampus, anterior cingulate cortex and pre-supplementary motor area. See....

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Results

Electrophysiology setup diagram and equipment; includes eye tracker, robotic arm, stimulus laptop.
Figure 1. Experimental setup. (A) The left panels show a sketch of the connections between the different systems. The stimulus computer serves as the central controller. It connects to the electrophysiology system through the parallel por.......