Recording CA3-CA1 Synaptic Responses in a Rat Hippocampal Slice

In This Article

Abstract

Source: Shetty, M. S. et al., Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents. J. Vis. Exp. (2015).

This video demonstrates a method to record CA3-CA1 synaptic responses in a rat hippocampal slice. It outlines the steps involved in placing the stimulating and recording electrodes on the hippocampal slice and measuring the field excitatory postsynaptic potential (fEPSP) to assess synaptic response strength.

Protocol

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.

1. Recording of CA3-CA1 Synaptic Responses

NOTE: The electrophysiology set-up used for field potential recording is shown in Figure 1A. A Faraday cage is strongly recommended if the electrical interference is beyond the control after the proper grounding of the electrical settings. Many different types of submerged and interface chambers are commercially available. However, interface chambers are preferred as slices exhibit more robust synapti....

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Results

Neuroscience experimental setups; images show electrophysiology workstation, microelectrode array.
Figure 1. Electrophysiology set-up for field-potential recordings consisting of (A) stimulators (b) a differential amplifier (c) an analog-to-digital converter (d) Oscilloscope (e) computer with acquisition software (f) Vibration-resistan.......