Whole-Cell Patch Clamp Recordings on Intact Rat Dorsal Root Ganglia

Abstract

Source: Gong, K.,et al. Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats. J. Vis. Exp. (2016)

This video demonstrates the examination of sensory neuron excitability in the dorsal root ganglia using the patch clamp technique to measure the rheobase and membrane potential, analyzing the neurons' responses to incremental current injections.

Protocol

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.  

1. Patch Clamp Recordings

1. Place the electrode solution (see Table 1) on ice to prevent degradation. Fill the glass patch pipette with filtered intracellular solution (syringe filter, pore size: 0.2 µm) and place the pipette in the head stage pipette holder. Apply gentle, positive pressure to the pipette through a 5 ml syringe by displacing the plunger about 1 ml before lowering the pipette into the bath solution.
   Note: Th....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Glass pipette	Sutter	BF150-110-7.5
Anchor	Havard apparatus	64-0250	stabilize the DRG to avoid drift.
Peristaltic pump	WPI
Pipette puller	Sutter	P97
Amplifier	Molecular devices	Axopatch 200B
Digitizer	Molecular devices	1440D
Microscope	NIKON	FN600
Micro-manipulator	Sutter	MPC200
Carbogen (95% O2, 5% CO2)	Local Medical Gas supplier