Implanting an Electrode Array and Optic Fiber into the Mouse Hippocampus

Abstract

Source: Bender, F., et. al., Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice. J. Vis. Exp. (2018)

This video demonstrates the stereotactic implantation of a wire electrode array and an optic fiber in the hippocampus of a mouse model.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of Optic Fibers (Figure 1A)

1. Use multimode optic fiber (105 µm core, glass-clad with silica core, 0.22 NA). Strip the 125 µm cladding of the fiber core using a micro-stripper while the fiber is still attached to the fiber spool.
2. Cut the fiber to a length of approximately 2-3 cm using a diamond knife.
3. Insert the fiber in a zirconia ceramic stick ferrule (ID: 126 µm). Approximately 0.5-1 mm of the optic fib....

Results

Figure 1: Illustration of optic fibers, electrodes and surgery. (A) Illustration of an optic fiber. (B) Illustration of a wire array glued to an optic fiber for the recording of hippocampal LFP during entrainmen.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
PV-Cre mice	The Jackson Laboratory	B6;129P2-Pvalbtm1(cre)Arbr/J
Stereotaxis	David Kopf Instruments, Tujunga, CA, USA	Model 963	Ultra precise small animal stereotaxic instrument
Drill bits, 0.8 mm	Bijoutil, Allschwil, Switzerland	49080HM
Soldering station	Weller Tools GmbH, Besigheim, Germany	WSD 81
Erythromycin	Rotexmedica GmbH, Trittau, Germany	PZN: 10823932	1g powder for solution for infusion
Precision drill/grinder	Proxxon, Wecker, Luxemburg	fbs 240/e
Multimode optic fiber	ThorLabs, Dachau, Germany	FG105LCA	0.22 NA, Low-OH, Ø105 µm Core, 400 - 2400 nm
Ceramic stick ferrule	Precision Fiber Products, Milpitas, CA, USA	CFLC126	Ceramic LC MM Ferrule, ID 126um
Polishing paper	Thorlabs	LF3D	6" x 6" Diamond Lapping (Polishing) Sheet
Power meter	Thorlabs	PM100D	Compact Power and Energy Meter Console, Digital 4" LCD
Multimode fiber optic coupler	Thorlabs	FCMM50-50A-FC	1x2 MM Coupler, 50:50 Split Ratio, 50 µm GI Fibers, FC/PC
Fiberoptic patch cord	Thorlabs	FG105LCA CUSTOM-MUC	custom made, 3 m long, with protective tubing, Tubing: FT030, Connector 1: FC/PC, Connector 2: 1.25mm (LC) Ceramic Ferrule
Sleeve	Precision Fiber Products, Milpitas, CA, USA	ADAL1	Ceramic Split Mating Sleeve for Ø1.25 mm (LC/PC) Ferrules
Tungsten wires	California Fine Wire Company, Grover Beach, CA, USA	CFW0010954	40 µm, 99.95%
Capillary tubing	Optronics	1068150020	ID: 100.4 µm
Omnetics nanoconnector	Omnetics Connector Corporation, Minneapolis, USA	A79038-001
Screws	Bilaney, Düsseldorf, Germany	00-96x1/16	stainless-steel
Silver conductive paint	Conrad electronics, Germany	530042
Liquid flux	Felder GMBH Löttechnik, Oberhausen, Germany	Lötöl ST	DIN EN 29454.1, 3.2.2.A (F-SW 11)