Induction of Elevated Intraocular Pressure in a Rat Model

Abstract

Source: Lani-Louzada, R., et al., Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents. J. Vis. Exp. (2022)

This video demonstrates the induction and monitoring of elevated intraocular pressure (IOP) in a rat model. The process involves cauterizing the limbal blood vessels, resulting in vessel occlusion and elevated IOP. The rat is then allowed to recover, and IOP is regularly monitored in both the experimental and control eyes for comparison.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

In the present work, Lister Hooded rats of both genders were used, aged 2-3 months and weighing 180-320 g. However, the procedure can be adapted in different rat strains of various age ranges.

Ocular hypertension surgery and clinical follow-up

1. House animals i....

Results

Figure 1: Thermal cauterization of limbal vascular plexus and consequences to retinal function in vivo. (A) Alternative methods to measure IOP in rats: rebound tonometry (superior), and applanation tono.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Cautery Low Temp Fine Tip 10/bx	Bovie Medical Corporation	AA00	Low-temperature ophthalmic cautery
Cetamin	Syntec do Brasil Ltda	000200-3-000003	Ketamine hydrochloride 10%
Ecofilm	Cristália Produtos Químicos Farmacêuticos Ltda	MS-1.0298.0487	Carmellose sodium 0.5%
Hyabak	União Química Farmacêutica Nacional S/A	MS-8042140002	Sodium hyaluronate 0.15%
Icare Tonolab	Icare Finland Oy	TV02 (model number)	Rebound handheld tonometer
Maxiflox	Cristália Produtos Químicos Farmacêuticos Ltda	MS-1.0298.0489	Ciprofloxacin 3.5 mg/g
Ster MD	União Química Farmacêutica Nacional S/A	MS-1.0497.1287	Prednisolone acetate 0.12%
Terolac	Cristália Produtos Químicos Farmacêuticos Ltda	MS-1.0497.1286	Ketorolac trometamol 0.5%
Terramicina	Laboratórios Pfizer Ltda	MS-1.0216.0024	Oxytetracycline hydrochloride 30 mg/ g + polymyxin B 10,000 U/g
Tono-Pen XL	Reichert Technologies	230635	Digital applanation handheld tonometer
	Carl Zeiss	-	Stereo microscope for surgery and retinal dissection