Fluorescence Imaging of Neuro-Immune Dynamics Using a Skull Implant

Abstract

Source: Bisht, K., et al. Precise brain mapping to perform repetitive in vivo imaging of neuro-immune dynamics in mice. J. Vis. Exp. (2020)

This video demonstrates fluorescence imaging of neuro-immune dynamics in a transgenic mouse with a skull implant. Vascular and neuronal stability is imaged alongside dynamic microglial changes, reflecting immune responses to neuronal activity.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Mouse preparation for cranial window implantation

NOTE: Various transgenic mouse lines with fluorescent tags are suitable for imaging.

1. Use CX3CR1GFP/+ mice to visualize microglia in vivo. Typically, juvenile to young adult 4 to 10-week-old mice that weigh ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Coverglass (3mm)	Warner Instruments	64-0726
Cyanoacrylate glue (Krazy Glue)	Amazon	https://www.amazon.com/Krazy-Glue-Original-Purpose-Instant/dp/B07GSF31WZ/ref=sr_1_2?keywords=krazy+glue&qid=1583856837&s=pet-supplies&sr=8-2
Demi Ultra LED Curing Light System	Dental Health Products, Inc	910860-1
Dental Drill	Osada: www.osadausa.edu	EXL-M40
Drill Bit	Fine Science Tools	#19008-07
Eye Ointment	Henry Schien	1338333
iBond Total Etch (Primer glue)	Chase Dental Supply (Heraeus Kulzer)	66040094
Rhodamine B	Millipore Sigma	81-88-9 (R6626)
Tetris Evoflow glue (Final glue)	Top Dent (Ivoclar Vivadent)	#595956
Wahl Brav Mini+	Amazon	https://www.amazon.com/Wahl-Professional-Animal-BravMini-41590-0438/dp/B00IN24ILE/ref=asc_df_B00IN24ILE/?tag=hyprod-20&linkCode=df0&hvadid=167141013968&hvpos=&hvnetw=g&hvrand=12368793083893626704&hvpone=&hvptwo=&hvqmt=&hvdev=c&hvdvcmdl=&hvlocint=&hvlocphy=9008337&hvtargid=pla-332197544154&psc=1