Intracerebroventricular Treatment with Resiniferatoxin for TRPV1 Desensitization in the Mouse Brain

Abstract

Source: Fukushima, A., et al. Intracerebroventricular Treatment with Resiniferatoxin and Pain Tests in Mice. J. Vis. Exp. (2020)

This video demonstrates the procedure for intracerebroventricular injection of resiniferatoxin in mice to desensitize the TRPV1 receptors in the brain.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Intracerebroventricular Injection of RTX

1. Anesthetize mice with pentobarbital sodium salt (60 mg/kg, intraperitoneally), and check for loss of the righting reflex.
2. For intracerebroventricular (i.c.v.) treatment, inject 5 µL of resiniferatoxin (RTX) (20 µg/mL) into the right lateral ventricle. For the control group, inject the vehicle (10% ethanol, 10% polyoxyethylene (20) sorbitan monooleate and 80% artificial cerebrospinal fluid (ACSF)) in the same way.
   1. Pass a disposable 27-G needle th....

Results

Figure 1: Photographic and schematic views of intracerebroventricular (i.c.v.) injecti.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Resiniferatoxin	LKT Laboratories	R1774	used for s.c./i.c.v. pretreatments and the RTX test
Pentobarbital sodium salt	Tokyo Chemical Industry	P0776	used for anesthesia
Ethanol (99.5%)	Wako Pure Chemical Industries	057-00456	used for dissolving RTX
Polyoxyethylene(20) Sorbitan Monooleate	Wako Pure Chemical Industries	161-21621	used for dissolving RTX
25 mL Microsyringe	Hamilton	1702LT	used for i.c.v. injection
26-gauge Disposable needle	TERUMO	NN-2613S	used for i.c.v. injection