Osmotic Pump-Based Neurotherapeutic Drug Delivery in a Demyelinated Mouse Model

Abstract

Source: Wang, X. et. al., Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System. J. Vis. Exp. (2021)

This video demonstrates the method for delivering neurotherapeutic drugs using an osmotic pump in a demyelinated mouse model. The controlled drug release promotes remyelination by activating myelin-producing cells in the corpus callosum.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Establishment of the lysolecithin-induced demyelination mouse model

1. Prepare 1% lysolecithin (also called L-α-Lysophosphatidylcholine) solution with sterile phosphate-buffered saline (PBS).
2. Sterilize scissors, forceps, curved hemostats, and other surgical instruments by autoclave sterilization. Sterilize the surgical area and lay down sterile sheets. All materials and reagents used for surgery should be prepared aseptically. It is important to keep the surgical area sterile throughout the procedure....

Results

Figure 1: Establishment of the lysolecithin-induced demyelination mouse model. (A) Secure the mouse in .......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Anesthesia air pump	RWD	R510-29	E05818-006
Brain infusion kit 3	ALZET	8851	1-3 mm
Carprofen	Macklin	C830557-1g	5 mg/kg every 24 h
Erythromycin eye ointment	Along technology	YCKJ-RJ-024780	Cover the surface of the eyeballs during anesthesia
Erythromycin ointment	pythonbio	RG180
Gas evacuation apparatus	RWD	R546W	E05518-002
L-α-lysophosphatidylcholine	Sigma	L0906	Dissolve at 1% with sterile PBS
Microliter syringe	Hamilton	65460-05	Syringe series:1700, 10 µL, 33 gauge
Micro-osmotic pump model 1002	ALZET	4317	0.25 µL per hour, 14 days
PBS (pH = 7.3)	ORIGENE	ZLI-9061
Pentobarbital sodium	Shanghai Civi	CAS NO: 57-33-0	150-200 mg/kg intraperitoneal injection for euthanasia
Small animal anesthesia machine	RWD	R520IE	E05807-006 M
Stereotaxic equipment	RWD	E06382
STERI 250 sterilizer	Keller	31101	Rapid sterilization of surgical instruments
Surgical sutures	Shanghai jinhuan	F504	5-0
Syringe needle (1 mL)	Shanghai KDL	6930197811018	26 gauge (0.45 mm x 16 mm)
Testing drug and solvent	Experiment dependent	N/A
Thermostar homeothermic monitoring system	RWD	69026	Maintain body temperature during anesthesia
Vetbond tissue adhesive	3M	1469SB	Secure the brain infusion cannula, and adhere the skin incision