High-Intensity Focused Ultrasound for Blocking Nerve Action Potentials in a Diabetic Rat Model

Abstract

Source: Lee, Y. F., et al. An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models. J. Vis. Exp. (2017)

This video demonstrates the use of high-intensity focused ultrasound (HIFU) to block action potentials from the sciatic nerve to the gastrocnemius muscle in a diabetic rat model. Upon delivering the HIFU beam to the nerve, a decrease in muscle signal confirms HIFU's potential as a non-invasive therapy for diabetic neuropathic pain relief.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. In Vivo Nerve Conduction Blockage with the High-Intensity Focused Ultrasound (HIFU) Transducer

NOTE: The in vivo experiment starts on week 5 after a 50 mg/kg streptozotocin (STZ) injection.

1. Perform animal procedures before blocking the compound muscle action potentials (CMAPs) with HIFU sonication.
   1. Sterilize the surgical tools (scalpel, scissors, forceps, and glass hook) in an autoclave before the sur....

Results

Figure 1: The Experimental Setup for In Vivo Nerve Conduction Block with High.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Streptozotocin	Sigma	85882
Biopac MP36 acquisition system	Biopac Systems, Inc.
HIFU transducer	Sonic Concepts	H108
Function generator	Agilent	33250A
Power amplifier	Electronics & Innovation	1040L
Rats	Biolasco taiwan	Sprague-Dawley
Puralube vet ointment	Dechra
Isoflurane vaporizer	Parkland Scientific	V3000PS
Isoflurane	Attane