Brain Tumor Resection in a Mouse Using a Minimally Invasive Resection System

Abstract

Source: Alomari, S., et al. Implementation of Minimally Invasive Brain Tumor Resection in Rodents for High Viability Tissue Collection. J. Vis. Exp. (2022)

This video demonstrates the use of a minimally invasive resection system (MIRS) to excise tumor tissue from an anesthetized mouse implanted with a tumor. The procedure involves securing the mouse onto a stereotactic frame, reopening the previous surgical site to access the brain, and inserting a specialized cannula that utilizes suction and a rotating blade to remove tumor sections. After resection, the excised tissue is collected through sterile tubing, and the wound is closed to allow for recovery.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Initial intracranial tumor implantation

1. At the initial phase of the study, intracranially inject each mouse with 100,000 cells (GL261 murine glioma cell line) suspended in 4 µL of phosphate-buffered saline (1x PBS) to a depth of 2.5 mm following the previously published report.
2. Quantify the tumor signal in each mouse using the in vivo imaging system 9 days following tumor implantation.
   NOTE: If needed, stratify the mice into two groups based on tumor burden. In the pre....

Results

Figure 1: MIRS system setup. (A) Minimally invasive resection system (MIRS.......

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Anased (Xylazine injection, 100 mg/mL)	Covetrus	33198
Anesthesia System	Patterson Scientific	78935903
Anesthesic Gas Waste Container	Patterson Scientific	78909457
C57Bl/6, 6-8 week old mice	Charles River Laboratories	Strain Code 027
Isoflurane	Covetrus	11695-6777-2
Isoflurane Vaporizer	Patterson Scientific	78916954
Ketamine	Covetrus	11695-0703-1
Kopf Stereotactic frame	Kopf Instruments	5001
Lightfield Microscope	BioTek	Cytation 5
Microinjection Unit	Kopf	5001
Micromotor drill	Foredom	F210418
MRI system	Bruker	7T Biospec Avance III MRI Scanner
NICO Myriad System	NICO Corporation
Ophthalmic ointment	Puralube vet ointment
Papain	Sigma Aldrich	#P4762
PBS	Invitrogen	#14190250
PenStrep	Millipore Sigma	N1638
Percoll solution	Sigma Aldrich	#P4937
Pipette controller	Falcon	A07260
Povidone-iodine solution	Aplicare	52380-1905-08
RPMI Media	Invitrogen	INV-72400120
Scalpel blade	Covetrus	7319
Scalpel handle	Fine Science Tools	91003-12
Skin marker	Time Out	D538,851
Staple remover	MikRon	ACR9MM
Stapler	MikRon	ACA9MM
Staples	Clay Adams	427631
Stereotactic Frame	Kopf Instruments	5000
Sucrose	Sigma Aldrich	S9378
Suture, vicryl 4-0	Ethicon	J494H