Assessing the Neuroprotective Effects of a Test Compound in a Middle Cerebral Artery Occlusion Mouse Model

Abstract

Source: Lee, S. E., et al. Assessing Neuroprotective Effects of Glycyrrhizae Radix et Rhizoma Extract Using a Transient Middle Cerebral Artery Occlusion Mouse Model. J. Vis. Exp. (2018)

This video demonstrates the procedure to assess the neuroprotective effect of a test compound in a middle cerebral artery occlusion mouse model. The compound enters the brain, prevents neuronal death, and reduces infarct volume, confirming its neuroprotective effect compared to an untreated mouse.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Mouse Model of Middle Cerebral Artery Occlusion (MCAO)

1. Use male C57BL/6 mice aged 6 weeks and weighing 22-25 g. Provide all animals with free access to standard chow and water, and house them in an environment with controlled temperature (22 ±1°C) and a 12 h light/dark cycle.
   1. Divide the mice into groups of six mice each, which should consist of sham-operated normal, control, and Glycyrrhizae Radix et Rhizoma (GRex) treatment groups.
   2. Perform MCAO ....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Glycyrrhizae Radix et Rhizoma	Gwangmyoung Pharmaceuticals Co., Korea
Stereo microscope	Leica	M50
Stereo microscope	Nikon	SMZ745
Laser Doppler	Moor Instrument	moorVMS-LDF
Anesthesia tabletop bracket with N₂O and O₂ flowmeter system	Harvard Appratus	34-1352
Homeothermic monitoring system	Harvard Appratus	55-7020
Mouse diet	Doo Yeol Biotech	Standard rodent chow
Isoflurane	JOONGWAE	A02104781
Isoflurane	TROIKAA	ISOTROY 100
Silk suture (4-0 black silk)	AILEE	SK47510
Silk suture (3-0 white silk)	Baekjae	57
Nylon suture (8-0 monofilament)	AILEE	NB825
Forcep	Fine Science Tools	11241-30
Forcep	Fine Science Tools	11254-20
Forcep	Fine Science Tools	11260-20
Fine forcep	Fine Science Tools	11274-20
Surgical scissors	Fine Science Tools	14001-12
Extra fine Bonn scissors	Fine Science Tools	14084-08
Moria Pascheff-Wolff spring scissors	Fine Science Tools	15371-92
Vessel dilating forcep	Fine Science Tools	18153-11