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rna extraction from neuroprecursor cells using the bio-rad total rna kit

RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

Hi, I'm Josh Sh, who I'm a graduate student at, at Dr.Ed Maki's lab at the University of California Irvine. I'll be showing you how to extract RNA from neuro precursor cells using this BioRad to RNA mini kit. I've been using this kit for a couple years already and it's at this lab and it's always been giving me consistent results at high yields.So the cells that we use are Eton and a half neuro precursor cells that have been acutely dissociated from embryonic cortices, and we plate them usually in a 24 well tissue culture treated to plate that has been PD L and laminate coated, and I plate them at a hundred thousand Cells per well. Before I start, I have to tell you that you should work on a, a designated bench where you do do your RNA work. First things first is to make sure your RNA bench is clean.Appropriate way to clean the bench is to use this RNA zap. Basically it kills off any RNAs reason being that the RA basically destroys your RNAs. Other thing is to wear gloves always when you're handling the extraction because your skin's filled with these RNAs.So it's a big no-no. Okay, so what I have here is the BioRad total RA mini kit. I've taken out its contents, so let me show you

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University of California, Irvine (UCI).  RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

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