March 2013: This Month in JoVE

Summary

Here are some highlights from the March 2013 issue of Journal of Visualized Experiments (JoVE).

Protocol

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Holly A. Robinson, SunKuk Kwon, Mary A. Hall, John C. Rasmussen, Melissa B. Aldrich, Eva M. Sevick-Muraca
Center for Molecular Imaging (CMI), University of Texas Health Science Center-Houston

Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.

Non-invasive Optical Measurement of Cerebral Metabolism and Hemodynamics in Infants

Pei-Yi Lin¹, Nadege Roche-Labarbe^{1, 2}, Mathieu Dehaes³, Stefan Carp¹, Angela Fenoglio³, Beniamino Barbieri⁴, Katherine Hagan¹, P. Ellen Grant³, Maria Angela Franceschini^1
1Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, ²Lab. PALM, Université de Caen Basse-Normandie, ³Fetal-Neonatal Neuroimaging and Developmental Science Center, Boston Children's Hospital, Harvard Medical School, ⁴ISS, INC.

We combined frequency-domain near-infrared spectroscopy measures of cerebral hemoglobin oxygenation with diffuse correlation spectroscopy measures of cerebral blood flow index to estimate an index of oxygen metabolism. We tested the utility of this measure as a bedside screening tool to evaluate the health and development of the newborn brain.

Reduction In Left Ventricular Wall Stress And Improvement In Function In Failing Hearts Using Algisyl-LVR

Lik Chuan Lee¹, Zhang Zhihong¹, Andrew Hinson², Julius M. Guccione^1
1Department of Surgery, UCSF/VA Medical Center, ²Clinical & Regulatory, LoneStar Heart, Inc.

This article describes procedures for implanting a novel hydrogel in failing hearts and quantifying its effect on left ventricular wall stress and function. These procedures have been successfully applied in dogs and humans.

Development Of An Audio-based Virtual Gaming Environment To Assist With Navigation Skills In The Blind

Erin C. Connors¹, Lindsay A. Yazzolino¹, Jaime Sánchez², Lotfi B. Merabet^1
1Laboratory for Visual Neuroplasticity, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, ²Department of Computer Science and Center for Advanced Research in Education (CARE), University of Chile

Determining 3D Flow Fields Via Multi-camera Light Field Imaging

Tadd T. Truscott¹, Jesse Belden², Joseph R. Nielson¹, David J. Daily¹, Scott L. Thomson^1
1Department of Mechanical Engineering, Brigham Young University, ²Naval Undersea Warfare Center, Newport, RI

A technique for performing quantitative three-dimensional (3D) imaging for a range of fluid flows is presented. Using concepts from the area of Light Field Imaging, we reconstruct 3D volumes from arrays of images. Our 3D results span a broad range including velocity fields and multi-phase bubble size distributions.