Compensatory Limb Use and Behavioral Assessment of Motor Skill Learning Following Sensorimotor Cortex Injury in a Mouse Model of Ischemic Stroke

Summary

Mouse models have become increasingly popular in studies of behavioral neuroscience. As models advance, it is important to develop sensitive behavioral measures specific to the mouse. This protocol describes the Pasta Matrix Reaching Task, which is a skilled motor task for use in mouse models of stroke.

Abstract

Mouse models have become increasingly popular in the field of behavioral neuroscience, and specifically in studies of experimental stroke. As models advance, it is important to develop sensitive behavioral measures specific to the mouse. The present protocol describes a skilled motor task for use in mouse models of stroke. The Pasta Matrix Reaching Task functions as a versatile and sensitive behavioral assay that permits experimenters to collect accurate outcome data and manipulate limb use to mimic human clinical phenomena including compensatory strategies (i.e., learned non-use) and focused rehabilitative training. When combined with neuroanatomical tools, this task also permits researchers to explore the mechanisms that support behavioral recovery of function (or lack thereof) following stroke. The task is both simple and affordable to set up and conduct, offering a variety of training and testing options for numerous research questions concerning functional outcome following injury. Though the task has been applied to mouse models of stroke, it may also be beneficial in studies of functional outcome in other upper extremity injury models.

Introduction

Mouse models have become increasingly popular for experimental stroke research due in part to their convenience and affordability, as well as the availability of transgenic lines that are suitable for in vivo imaging among other applications. With this increased popularity in experimental models, the interest in developing sensitive behavioral assessments of functional outcome following injury has also increased ^1-7. The development of animal training protocols that mimic both rehabilitation and compensatory strategies used by human stroke survivors improves the ability to successfully translate findings from experimental animal studies to use in the clinic ⁸. Motor skill training on the Pasta Matrix Reaching Task (PMRT) has been previously established as a sensitive behavioral assessment of motor skill outcome following ischemic insult of the sensorimotor cortex ³.

One of the primary interests in stroke research concerns rehabilitation and the development and understanding of behavioral strategies that promote improved recovery of function following insult. Currently, rehabilitation strategies in humans result in incomplete recovery ⁸. In addition, rehabilitation therapists must combat compensatory strategies that stroke survivors develop during recovery that may undermine their ability to fully regain function of their affected limb(s). For example, following a unilateral stroke that affects upper extremity function, humans tend to develop a reliance on their less-affected limb ^{9, 10}. While improving a person’s ability to function in the short term, this learned non-use of the affected limb may impede its ultimate recovery potential, as demonstrated in animal models ^11-13. These findings in animals have helped to inform the development and use of constraint-induced movement therapy in humans ¹⁴. Animal models are beneficial for improving rehabilitation strategies by allowing researchers to explore the neurobiological mechanisms that subserve and promote recovery of function. In addition to being an effective behavioral assessment of post-stroke function, the PMRT has been established as an effective rehabilitative strategy to promote improved functional outcome following sensorimotor stroke ¹⁵. The PMRT can also be used to effectively mimic learned non-use of the affected limb and therefore offer insight into behavioral manipulations that may improve functional recovery despite initial over-reliance on the less-affected limb ¹³.

Construction of the PMRT has been described previously ³. Briefly, the reaching chamber is composed of four Plexiglas walls (20 cm tall, 15 cm long, and 8.5 cm wide) with an open top and bottom. There is a center slit (13 cm tall and 5 mm wide) extending from the bottom base of the front wall of the chamber that serves as the reaching aperture (Figure 1A). The pasta matrix is a heavy-duty plastic block (8.5 cm long, 5 cm wide, and 1.5 cm tall) with 1 mm diameter holes drilled completely through the depth of the block. There are a total of 260 holes, beginning 2 mm from the reaching window with 2 mm between each hole (Figure 1B). The pasta matrix is designed such that dry, vertically oriented pasta pieces extend through the entire depth of the matrix stage with approximately half of the pasta piece exposed. A removable piece of overhead plastic or cardstock should be cut to size and taped securely to the underside of the matrix. This prevents the pasta pieces from falling out of the matrix during transport and allows for easy removal of broken pasta pieces.

The PMRT is a versatile and sensitive behavioral assay that permits experimenters to collect accurate outcome data and manipulate limb use to mimic clinical phenomena. As a behavioral outcome measure, the PMRT allows experimenters to collect behavioral data that more accurately reflect the effectiveness of a rehabilitative strategy than does the traditional measure of infarct size ^{3, 16}. As a behavioral manipulation, the PMRT allows experimenters to control upper limb use in mice in order to mimic clinical experiences of rehabilitation (i.e. affected limb training) or learned non-use (i.e. less-affected limb training). When combined with neuroanatomical methods, the PMRT provides researchers with an opportunity to explore the mechanisms that support behavioral recovery of function or maladaptive plasticity following compensatory limb use after stroke. The PMRT could be further applied to other murine models of brain injury and upper extremity impairment, such as traumatic brain injury. Another advantage of the PMRT is its affordability. The equipment required for the task can be constructed fairly reasonably in house, data collection does not require a large amount of space or financial resources, and the task is simple enough for undergraduate students to reliably collect data. Further, the PMRT is sensitive to even small behavioral deficits ^{3, 13}. This protocol provides a simple and effective way to assess motor skill learning, promote behavioral recovery following injury, and mimic learned non-use phenomena in an established murine model of stroke.

Protocol

The following methods are in accordance with protocols approved by the University of Texas at Austin and Illinois Wesleyan Animal Care and Use Committees. It is recommended that researchers either wear gloves or take appropriate precautions (washing hands before and after) when engaging in behavioral training with any laboratory animal. Gloves must be worn when handling animals in preparation for and during surgery.

1. Habituation and Food Restriction

NOTE: As the PMRT is an appetitive task, it is beneficial to restrict the amount and timing of feeding prior to the onset of training in order to motivate the reaching response and prevent satiety during training sessions.

1. Place mice on a restricted feeding schedule beginning at least 1 week prior to the onset of task training. Weigh each mouse prior to the onset of food restriction to establish free-feeding weights, which are determined as the weights of each animal on the first day of restricted feeding.
   1. Feed mice once daily, with each mouse receiving ~2.5 g of standard rodent chow per day (e.g., if there are 4 mice housed in a cage, the cage will receive 10 g of food daily). Ensure feeding occurs at the same time each day, preferably at the time daily training sessions are expected to conclude.
   2. Weigh animals regularly to ensure that no mouse loses more than 10% of their free-feeding weight. NOTE: Adjust daily food amounts accordingly to promote appropriate weight maintenance. Mice typically gain weight as they mature, even with constant food restriction Mice remain on food restriction for the duration of PMRT training/assessment.
2. Expose animals to smaller pieces of pasta (cut to 1.6 cm) in their home cage several times prior to training in order to overcome neophobic responses. NOTE: Typically 5-10 days of exposure are sufficient, with about 4 pieces per mouse provided at each feeding.
3. Cut pasta pieces to size (3.2 cm) using a razor blade. NOTE: It is recommended that an excess of pasta pieces are cut prior to beginning the day’s training and unused pieces stored in an airtight container. Capellini pasta is used to fill the matrix. As different brands of pasta vary in texture and diameter, which could influence the stability of pasta in the matrix or the amount of force necessary for the mice to break the pasta, it is recommended that DeCecco brand pasta be used throughout training and testing.
4. Place uncooked capellini pasta pieces in the pasta matrix, filling all available holes in the matrix (Figure 1A).
5. Habituate mice to the testing chamber with a full pasta matrix placed in front (Figure 1A) and several pieces of pasta to eat at the bottom of the testing chamber. Habituate mice for 3 sessions of 5-10 min. NOTE: Mice should not be encouraged to reach for pasta during habituation training. Mice usually begin to show interest in the pasta located outside of the reaching chamber by sniffing at it through the aperture. It is possible to habituate two cage mates in a single chamber if desired, but mice should be shaped and trained individually. All surfaces should be cleaned with 70% ethanol or a similar, safe disinfecting solution before and after each animal is placed in the behavioral apparatus.

2. Shaping the Reach

1. Determine each animal’s preferred limb by shaping the reach over 3-5 once-daily shaping trials. Permit mice to reach for 10 min or a total of 10 times, which occurs first.
   1. Place mice individually in the testing chamber with a full pasta matrix in front of the chamber as in habituation training (Figure 1A).
   2. Record the number of reaches with each limb, denoting the number of reaches with the right and left limbs separately. Define a reach as extension of the limb through the reaching aperture such that the wrist breaks the plane of the chamber; it is not necessary for the reach to be directed toward or make contact with the pasta.
2. Hold several pieces of pasta just inside the chamber through the reaching aperture to encourage mice that are reluctant to reach. Draw the pasta pieces out when the mouse shows interest (e.g. sniffing, biting), thus encouraging them to reach after the retreating pasta pieces. If the mice do not initially show interest in the dry pasta, then wet the tips of the pasta pieces in a small cup of warm water to make them more palatable.
3. Determine limb preference when a mouse exhibits a minimum of 70% of their daily reaches with a single limb. Do not to over-shape animals, as this can limit the amount of learning observed during the training portion of reaching tasks ¹⁷. Begin PMRT training once mice are shaped. NOTE: Training can be started as soon as the next day, but it is also acceptable to wait several days between shaping and training procedures.

3. Reaching Training

1. Train mice once daily prior to insult in order to establish proficiency on the PMRT. NOTE: Each trial consists of a maximum of 15 min or 100 reaches counted by the observer (successful and unsuccessful), whichever occurs first. Mice are trained to reach only with their preferred limb. Mice will tolerate two trials per day if a more intense training paradigm is desired ¹⁸.
   1. Place mice in the testing chamber with a half-full matrix placed in front (Figure 1B). Train mice to reach only with their preferred limb by filling only the side of the matrix contralateral to the preferred limb since mice reach across the midline of their body. Leave the portion of the matrix corresponding to the unpreferred limb unfilled in order to discourage reach attempts with this limb (which would not be successful because they cannot reach the pasta) and encourage reaching only with the preferred limb.
2. Place a small amount of pressure on the pasta while it is in the matrix to help mice successfully break pieces during early training sessions. Do not break the pasta pieces for the mouse. Achieve this by holding one or two pasta pieces lightly behind and perpendicular to the intended reaching target. This small amount of support helps stabilize the thin pasta, which is quite flexible and difficult for mice to grasp as they are initially learning the task.
3. Record the total number of reaches, number of successful reaches, and locations of successful reaches. Successful reaches require the mouse to reach through the aperture, grab a piece of pasta, and break the pasta piece to remove it from the matrix. NOTE: Pasta pieces should not be replaced in the matrix until after the trial has terminated. Early in training, mice will not break many pasta pieces.
4. Train mice on the PMRT to proficiency, which is defined as at least 3 days (and up to 5 days) of consistent performance (breaking at least 9 pieces of pasta with no more than 2 more or fewer pieces over days). It often takes between 15-20 days to train young adult mice to proficiency.

4. Less-affected Limb Training

1. Train the less-affected limb (i.e., the pre-operative unpreferred limb) beginning four days after ischemic insult to the motor cortex contralateral to the preferred reaching limb (see Figure 2 for clarification on affected/preferred and less-affected/unpreferred limbs). Administer once daily 15-min or 100-reach training sessions.
   1. Remove the first column of pasta (i.e., the most medial vertical column extending from the reaching aperture) for the first week of less-affected limb training in order to ensure that early reaches with the affected limb are not successful.
   2. Place mice in the reaching chamber with the half-filled matrix placed in front. Ensure that the filled half of the matrix is opposite of the pre-operative filled half, which will force the mice to now reach with the less-affected (unpreferred) limb.
2. Encourage reaching with the less-affected limb by reinforcing extension of this limb through the reaching aperture. When mice extend the less-affected limb in the first few days of training place a small (1/2 piece) of pasta onto the floor of the reaching chamber regardless of whether or not the reach is successful.
3. Count only those reaches made with the less-affected limb toward to the 100 reach total. Do not count furtive attempts with the affected limb towards the total number of reaches.
4. Return the most medial vertical column of pasta to the matrix after the first 7 days of less-affected limb training.
5. Record the number of reaches, number of successful reaches, and locations of successful reaches. NOTE: These data can be analyzed to observe differences in reaching patterns with the less-affected limb, but have not been previously reported.

5. Reaching Analysis

Determine the impact of less-affected limb use on recovery of function of the affected limb by assessing the affected limb on the PMRT task following less-affected limb training. This assessment occurs after the desired number of less-affected limb training days are complete (typically 14 days).

1. Place the matrix in front of the chamber, oriented identically to training procedures (i.e., with the half of the matrix contralateral to the preferred limb filled). NOTE: This forces mice to reach with their affected (and previously preferred) limb.
2. Record the total number of reaches, number of successful reaches, and locations of successful reaches to compare with pre-stroke performance levels. Do not probe performance of the affected limb during less-affected limb training as these affected limb probes may function as training sessions that could impact recovery of the affected limb.

Results

The results from PMRT analysis should include the number of pasta pieces broken and the pattern of successful reaches. Results from mice with sensorimotor cortical lesions indicate that ischemic insult affects both the number of successful reaches as well as physical reach patterns ^{3, 19}, as demonstrated in Figure 3A. Representative pattern changes can be observed in Figure 3B. The ability of the PMRT to mimic learned non-use effects in mice is demonstrated by impaired recover...

Discussion

The PMRT represents a simple, quantitative way to assess skilled reaching performance in mice. Though time consuming, it is possible for previously inexperienced undergraduates to be trained to collect reliable and reproducible data with only a few training sessions. The task is sensitive enough to measure even slight changes in mouse motor skill performance following ischemic insult ^{3, 13, 15}, and a number of studies cite long-term deficits with a variety of training protocols ^{15, 20, 22}. Because i...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Reaching chamber			Reaching chambers are made in house with Plexiglas
Pasta matrix block			The Pasta matrix box is made in house using a heavy plastic block
Capellini pasta	DeCecco		DeCecco brand capellini pasta can be purchased in a grocery store or through an online retailer such as Amazon