A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine

Summary

The use of a simple device to cut and ‘roll’ mouse intestines to rapidly prepare whole mount preparations is described.

Abstract

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe the use of a simple device to cut and ‘roll’ mouse intestines to rapidly prepare whole mount preparations of superior and uniform quality to that which can be achieved by hand. The device comprises a base that holds 4 stainless steel rods and a top, which acts a cutting guide. The rods are inserted into the lumen of the small intestine [divided into thirds] and the colon. The rods and samples are then placed over a piece of filter paper or card into the holding slots in the base of the device. The top of the device is then positioned and serves as a cutting guide. The two angled sections in the center of the top piece are used to guide a knife or scalpel and cut the intestines longitudinally on the top of the rods. Once the intestinal sections have been cut, the top is removed and the card, tissue and rods gently removed from the device and placed on the bench. The rods are then gently rolled sideways to flatten and stick the intestinal segments onto the underlying piece of filter paper or card. The final preparation can then be examined or fixed and stored for later analysis. The preparations are invaluable for the study of intestinal changes in normal or genetically modified mouse models. The preparations have been used for the study and quantification of the effects of inflammation (colitis), damage, pre-cancerous lesions (aberrant crypt foci (ACFs) and mucin depleted foci (MDFs)) and polyps or tumors.

Introduction

The mouse and the genetically modified mouse^1-3 are an invaluable model for the study of various pathologies especially cancer and its relationship to inflammation in the gastrointestinal tract. This often requires the preparation of whole mounts of the small intestine and/or colon.

The preparation of whole mounts can be done with a precision pair of offset scissors but it can take about 20 min per mouse⁴. This is not very practical for comparative studies, which require a significant amount of mice (say 10 per group subject to statistical analysis). In addition, the length of time taken increases the risk of tissue degradation. An attempt to alleviate this problem led to the trial of various cutting preparation aids. The first was based on a series of metal plates, with cutting groves, but the plates obscured the tissue and the cutting was thus difficult to control.

A conventional six-sided pencil finally provided inspiration for the cutting device, as we could then see how a half pencil shape would make a good guide for a scalpel (Figure 1). This resulted in a design for a frame with four cutting guides⁵. The effectiveness of this device was dramatic as the preparations in Figure 4 demonstrate the difference between the scissor preparation and the device preparation.

The tops of the original devices was constructed from several pieces of metal, but later ones, and the current model was machined out of a solid piece of Duralumin. The base was machined out of highest quality high-density resin.

The device has proved to be of great use for the preparation of whole mounts of mouse intestine as it greatly speeds up and improves the quality and consistency of the preparations, which have a wide range of applications.

Protocol

The various experimental procedures and the humane euthanasia are approved by the host institutions ethical committee and by the appropriate regulatory bodies. Euthanasia is normally achieved using CO₂ asphyxiation followed by cervical dislocation.

1. Device

1. Machine the main portion of the base of the device from out of a solid block of high-density acetyl resin, as described in this manuscript. At each end create 3 mm high-elevated strips, and part machine out of these 2.5 mm groves to take the rods (see Figure 2 and Figure 3).
2. Make rods from a 2.4 mm diameter stainless steel with rounded-off ends.
   NOTE: The main frame of the lid was machined out of Duralumin plate. A limited number of finished devices are available from Dr. Goodlad.

2. Use of the Device

1. Dissect out the small intestine and colon from the abdominal cavity using scissors and gloved fingers. Remove the mesentery by holding it with curved forceps and gently pulling it away from the intestine.
2. Rinse the intestines with cold phosphate buffered saline (PBS, pH 7.4) using a Gilson type pipette tip, which had been cut down at its wider end so that it can fit onto a 10 or 20 ml luer fitting plastic syringe.
3. Lay out the small bowel (SB) on to a sheet of paper towel and divide into three equal sections in length (proximal, mid, and distal – SB1, SB2 and SB3) using scissors. Remove the cecum and lay out the colon.
4. Make several very small cuts into the intestinal segments with scissors to allow the fluid to escape and place a sheet of paper towel over the intestines and gently run a finger over the preparations to squeeze out the fluid and blot dry.
5. Lift the sections and insert the stainless steel rods that were previously wetted by soaking in a beaker of phosphate buffered saline.
   NOTE: The intestine can be inverted if so desired (turned inside out).
6. Label a piece of card or filter paper cut to size to fit into the base of the device. Pencil is best, as it is unaffected by virtually all solutions. Autopsy date, experimental code and animal identification number are suggested.
7. Place the card or filter paper into the base of the device. Insert the rods and intestines into the slots in the base of the device. Make sure the proximal end of the segments is positioned in a standardized manner. The proximal ends should be near the card labels.
8. Place the top piece of the device over the base. Use the angled bars to guide a scalpel blade to longitudinally cut the sections.
   NOTE: It helps if the tissue is gently and carefully held with a finger to ensure that it does not move with the knife.
9. Remove the top piece of the device. Carefully remove the filter paper and tissues (still on their rods).
   NOTE: A piece of stiff card placed underneath the filter paper will aid lifting.
10. Slightly wet (with a gloved fingertip dipped in PBS) the segments. Roll the rod, side to side to open up the gut and spread it flat. The tissue will then adhere to the filter paper.
11. Visually examine the preparations for any gross lesions.
12. Transfer the tissue adhered to the filter paper to a shallow bath (or sandwich box) containing fixative.
    NOTE: After the tissue adheres to the filter paper or to card it will remain firmly attached. A wide range of fixatives can be used to preserve the preparation. It is recommended that Carnoy’s fluid should be used for the study of intestinal cell proliferations⁶, which is the ideal fixation for the best cell proliferation methods; however formalin or many other fixatives can also be used if other endpoints are required.
13. After fixation (usually 3 hr), transfer the preparations into 70% ethanol. The samples can then be stored until required. The preparations can be studied en face and/or used for the preparation of histological samples for H&E or other histological staining techniques, immunohistochemistry and/or in situ hybridization.
    NOTE: Prolonged fixation can reduce immunohistochemical reactivity and/or in situ hybridization reactivity. Polypropylene sandwich boxes are ideal for storing a large number of the preparations.
14. If required the fixed tissues from the various intestinal segments can be made into ‘Swiss rolls’ and stained with the entire range of histological methods⁶ including those described above. To make a ‘Swiss roll’ the intestinal segments are removed from the filter paper, rolled around a cocktail stick or a toothpick, secured with an entomological pin and processed for wax embedding⁶.
15. If required, stain the bulk tissue with methylene blue to visualize aberrant crypt foci⁷.
16. Score the fixed preparations at leisure.

Results

Polyps are easy to identify under a stereomicroscope with a cold light source to side illuminate the tissue (Figure 4). It is helpful to mark the card with pencil marks when a polyp is observed in order that scoring can be discussed later and different operators can reach agreement. The tissue can also be bulk stained [with methylene blue] to identify aberrant crypt foci⁸ or mucin depleted foci (MDFs)⁹ by illumination from above or by trans illumination (Figure 5).<...

Discussion

The device greatly speeds up the process so that two operators can fully autopsy a mouse within 6 min. This will include a visual check for obvious gross lesions and weighing and fixing the major organs. It will also allow the operators to rinse, blot and weigh the stomach, caecum, small intestine and colon and prepare the whole mounts. The flattened tissue can be fixed very quickly and thus avoid degradation artifacts.

One of the major advantages of the device (apart from speed) is that the d...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Delrin	Ryan Plastics, Earls Barton, UK		High-density acetal resin similar material would suffice
Duralumin plate	Metal Supplies Ltd, Park Road,Dukinfield. SK16 5LP UK		Aluminium allow dating back to 1909 so alterative suppliers are available
Finished device(s) ready for use	Contact Dr Goodlad  r.goodlad@imperial.ac.uk		Contact  r.goodlad@imperial.ac.uk for supply details