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Errata

Erratum: Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

doi: 10.3791/5617 Published: November 5, 2018

Abstract

A correction was made to Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. In Protocol section 1.1, the order of the listed chemicals MeOH:H2O2:DMSO has accidentally been switched and should instead be MeOH:DMSO:H2O2.

Protocol section 1.1 was changed from:

Incubate the tissue in freshly prepared MeOH:H2O2:DMSO bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

to:

Incubate the tissue in freshly prepared MeOH:DMSO:H2O2 bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

Protocol

A correction was made to Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. In Protocol section 1.1, the order of the listed chemicals MeOH:H2O2:DMSO has accidentally been switched and should instead be MeOH:DMSO:H2O2.

Protocol section 1.1 was changed from:

Incubate the tissue in freshly prepared MeOH:H2O2:DMSO bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

to:

Incubate the tissue in freshly prepared MeOH:DMSO:H2O2 bleaching buffer in a 2:1:3 ratio at RT for 24 hr to quench endogenous tissue fluorescence. For larger samples, exchange to new bleaching buffer and incubate for another 24 hr.

Disclosures

No conflicts of interest declared.

DOI

Cite this Article

Erratum: Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. J. Vis. Exp. (141), e5617, (2018).More

Erratum: Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. J. Vis. Exp. (141), e5617, (2018).

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