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Single Sensillum Recordings for Locust Palp Sensilla Basiconica

Published: June 23, 2018 doi: 10.3791/57863


This paper describes a detailed and highly effective protocol for single sensillum recordings from the sensilla basiconica on the palps of insect mouthparts.


The palps of locust mouthparts are considered to be conventional gustatory organs that play an important role in a locust's food selection, especially for the detection of non-volatile chemical cues through sensilla chaetica (previously named terminal sensilla or crested sensilla). There is now increasing evidence that these palps also have an olfactory function. An odorant receptor (LmigOR2) and an odorant-binding protein (LmigOBP1) have been localized in the neurons and accessory cells, respectively, in the sensilla basiconica of the palps. Single sensillum recording (SSR) is used for recording the responses of odorant receptor neurons, which is an effective method for screening active ligands on specific odorant receptors. SSR is used in functional studies of odorant receptors in palp sensilla. The structure of the sensilla basiconica located on the dome of the palps differs somewhat from the structure of those on the antennae. Therefore, when performing an SSR elicited by odorants, some specific advice may be helpful for obtaining optimum results. In this paper, a detailed and highly effective protocol for an SSR from insect palp sensilla basiconica is introduced.


Animals have evolved a range of chemosensory organs that sense exogenous chemical cues. In insects, the most important chemosensory organs are the antennae and the palps. On these organs, several types of chemosensory hairs, called chemosensory sensilla, are innervated by chemosensory neurons (CSNs) within the hairs. CSNs in chemosensory sensilla recognize specific chemical cues through signal transduction from chemical stimuli to electrical potentials that are subsequently transferred up to the central nervous systems1,2,3.

CSNs express various chemosensory receptors [e.g., odorant receptors (ORs)], ionotropic receptors (IRs), and gustatory receptors (GRs) on their membranes, which encode exogenous chemical cues associated with different types of chemosensation4,5,6. The characterization of CSNs is key to the elucidation of cellular and molecular mechanisms of insect chemoreception. Now single sensillum recording (SSR) is a widely-used technique for the characterization of insect CSNs in the antennal sensilla of many insects, including flies7, moths8, beetles9, aphids10, locusts11, and ants12. However, few studies have applied an SSR to insect palps13,14,15,16,17, because the particular structures of their sensilla make an electrophysiological recording difficult18.

Swarms of locusts (Orthoptera) often cause serious crop damage and economic loss19. The palps are believed to play an important role in the food selection of locusts20,21,22,23,24. Two types of chemosensory sensilla are investigated by a scanning electron microscope (SEM). Usually, 350 sensilla chaetica and 7 - 8 sensilla basiconica are observed on each dome of the locust palps18. Sensilla chaetica are gustatory sensilla that sense non-volatile chemical cues, whereas sensilla basiconica have an olfactory function, sensing volatile chemical cues.

On locust palps, the diameters of the hair sockets of the sensilla basiconica (ca. 12 µm), are much greater than those of sensilla chaetica (ca. 8 µm)18,25. The cuticular wall of the sensilla basiconica on the palps is much thicker than that of antennal sensilla18. In addition, the dome of the palp has fluid contents within a highly flexible cuticle. These characteristics mean that a penetration with a microelectrode and an acquisition of good electrophysiological signals is more difficult than for antennal sensilla. In this paper, a detailed and highly effective SSR protocol for locust palp sensilla basiconica is presented with a video.

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1. Preparation of Instruments and Insect

  1. Preparing tungsten electrodes and stimuli solutions
    1. Fix a new tungsten wire (diameter of 0.125 mm, length of 75 mm) into a micromanipulator and sharpen it in a 10% (w/v) sodium nitrite (NaNO2) solution in a syringe at 10 V provided by a power supply for about 1 min under a stereomicroscope (40X magnification).
    2. Dip the sharpened tungsten wire repeatedly into the 10% NaNO2 solution, about 4 mm at 5 V in < 1 min (Figure 1A).
    3. Examine the diameter of the sharpened tungsten tip frequently under the stereomicroscope until it is fine enough to penetrate the cuticle of a locust palp olfactory sensillum (Figure 1B).
    4. Prepare the stimulus solutions. Dilute each of the chemical stimulus substance in mineral oil. Dilute 1-nonanol and nonanoic acid at 10% dilutions. Dilute E-2-hexenal and hexanal at 10-2, 10-3, 10-4, and 10-5.
    5. Prepare Pasteur tubes carrying the stimuli: insert filter paper strips (length of 2 cm, width of 0.5 cm) into the Pasteur tubes, add the diluted stimulus solutions (each 10 µl) to the filter paper strips, and then plug the Pasteur tubes with pipette tips (1 ml).
  2. Prepare the insect
    1. Rear locusts (Locusta migratoria) with fresh wheat seedlings under crowded conditions at a relative humidity of 60%, a temperature of 28 - 30 °C, and a photoperiod of 18:6 h (light:dark). Choose 1- to 3-day-old 5th instar locust nymphs and remove the antennae with fine scissors to avoid any interference when recording.
  3. Preparing the locust maxillary palp holder
    1. Use a glass slide (25 mm x 75 mm) as the base of the maxillary palp holder (MPH). Attach a plastic piece (1 mm in height, 10 mm in width, 35 mm in length) to a corner of the glass slide with double-sided adhesive tape, and finally fix a cover glass (18 mm x 18 mm) on top of the plastic piece with double-sided adhesive tape. Place a small piece of red rubber tape onto the cover glass as a non-slip layer. The plastic piece and the cover glass constitute the platform for the locust palp. The height of the platform is approximately 1.5 mm.
    2. Install a tungsten wire (diameter of 0.125 mm, length of 36 mm) at a distance of 1.5 mm parallel to the inside edge of the platform. Fix the two ends of the wire onto the platform with double-sided adhesive tape.

2. Preparation of Locust Maxillary Palps

  1. Cut a centrifuge tube (1.5 ml) vertically in half and cut off the bottom. Place the locust into the prepared tube. Leave the ventral region and the head of the locust exposed. Fix the assembly to the glass slide with double-sided adhesive tape (Figure 2A).
  2. Pull the right maxillary palp onto the platform.
  3. Put the tungsten wire at the fourth segment of the palp. Place adhesive putty on each side of the tungsten wire, about 2 mm from the maxillary palp (Figure 2A and 2B).

3. Single Sensillum Recordings

  1. Place the locust maxillary palp preparation under a microscope at a low magnification (100X). Adjust the position of the preparation until the palp is perpendicular to the recording electrode (Figure 3A).
  2. Insert the reference electrode (tungsten electrode) into the locust eye using a micromanipulator. Move the recording electrode (tungsten electrode) close to the maxillary palp with the micromanipulator (Figure 3B and 3C).
  3. Adjust the odor delivery device to about 1 cm from the maxillary palp (Figure 3B).
  4. Open the recording software Auto Spike 32. Set the recording parameters as follows: the recording scale on 500 µV; the high cutoff of the filter on 300 Hz, the low cutoff on 200 Hz; and the pretrigger on 10 s.
  5. Connect the recording electrode to a 10x universal AC/DC amplifier.
  6. Switch the microscope to a high magnification (500X). Insert the recording electrode into the base of a basiconic sensillum on the maxillary palp and delicately adjust the recording electrode to obtain good spontaneous spikes (Figure 3D).
  7. Open the stimulus controller to deliver a continuous air stream at 20 ml/s. Set the stimulation time to 1 s. Record signals for 10 s, starting 10 s before the onset of the stimulus pulse.
  8. Use a 10x universal AC/DC amplifier to amplify the signals. Feed the signals into the IDAC 4. Analyze the signals with the Auto Spike 32 software. AC signals are band-pass filtered between 200 to 300 Hz. Use Auto Spike 32 to distinguish peak-to-trough amplitudes from noises. Calculate the responses of the neurons as the increases in action potential frequencies (spikes per second) over the spontaneous frequencies. Perform a statistical analysis using GraphPad Prism 7.

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Representative Results

Two sensilla subtypes (pb1 and pb2) on the locust maxillary palp are identified based on different response dynamics to chemical odorants (10% 1-nonanol and 10% nonanoic acid). The neurons in pb1 produce significantly more spikes to 1-nonanol than to nonanoic acid while the neurons in pb2 are significantly less activated by 1-nonanol compared with nonanoic acid (Figure 4). Hexanal and E-2-Hexenal can evoke a locust palp opening response (POR)26. Hexanal is an abundant host plant green leaf volatile which may contribute to a further confirmation to the food source26. The spikes elicited in the pb1 neurons last longer than those of pb2 when they are stimulated by E-2-hexenal (Figure 4). The neurons in pb1 and pb2 exhibit similarly robust responses to hexanal (Figure 4). Comparing the mean changes of all spikes between the periods 5 s before and 5 s after the stimulation indicates that the response to 1-nonanol is significantly higher than to nonanoic acid in pb1, but contrarily in pb2 (Figure 5). The neurons in these two subtypes of sensilla respond dose-dependently to E-2-hexenal and hexanal, and their response patterns to these two aldehydes differ (Figure 6A and 6B).

Figure 1
Figure 1. Electrode preparation. (A) This panel shows a general view of the electrode sharpening apparatus. The syringe containing 10% NaNO2 (left) is used to sharpen the electrode (right). (B) This panel shows a close view of the electrode tip (a: suitable; b: unsuitable). Please click here to view a larger version of this figure.

Figure 2
Figure 2. Locust maxillary palp holder (MPH). (A) The MPH and a locust are mounted on the glass slide before positioning it under the microscope. (B) This panel shows a close-up of the locust maxillary palp, fixed by tungsten wire on the platform. Please click here to view a larger version of this figure.

Figure 3
Figure 3. Single sensillum recordings. (A) This panel shows a view of the electrophysiology setup. (B) This panel shows a close view of the locust preparation mounted on the microscope. (C) This image shows the locust maxillary palp at 100X magnification. (D) This image shows the palp at 500X magnification. The arrow indicates a basiconic sensillum. Please click here to view a larger version of this figure.

Figure 4
Figure 4. Response traces of single sensillum recordings of the locust maxillary palp. In this panel, pb1 stands for subtype 1 of the palp sensilla basiconica; pb2 stands for subtype 2 of the palp sensilla basiconica. The bars above the traces indicate the stimulus duration (1 s). For these recordings, all odors are used at 10% dilutions except for E-2-hexenal and hexanal, which are diluted to 1%. This figure has been modified from Zhang et al.26. Please click here to view a larger version of this figure.

Figure 5
Figure 5. Comparison of mean numbers of spikes in neurons in pb1 and pb2 stimulated by nonanoic acid and 1-nonanol. The mean numbers of the spikes are calculated in the periods 5 s before and after stimulation. In pb1, the mean numbers of the spikes in the neurons responding to 1-nonanol increase significantly higher than those of the spikes in neurons responding to nonanoic acid (n = 11 palps; ANOVA with post hoc t-tests; p < 0.0001), in contrast to pb2 (n = 10 palps; ANOVA with post hoc t-tests; p = 0.0110). The error bar represents SEM. This figure has been modified from Zhang et al.26. Please click here to view a larger version of this figure.

Figure 6
Figure 6. The patterns of neurons in pb1 and pb2 responding dose-dependently to E-2-hexenal and hexanal. (A) This panel shows the patterns of the neurons in pb1 (± SEM; n = 12 palps). (B) This panel shows the patterns of the neurons in pb2 (± SEM; n = 10 palps). This figure has been modified from Zhang et al.26. Please click here to view a larger version of this figure.

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Insects rely on palps to detect food odors, and their palps are believed to play an important role in speciation13,27. The palps are simple olfactory organs and are receiving increasing attention as an attractive model for the exploration of the neuromolecular networks underlying chemosensation28.

Insect labellar and palp SSRs have been successfully performed on Drosophila melanogaster, Anopheles gambiae, and Culex quinquefasciatus13,14,15,16,17 but have rarely been reported in the form of a video presentation16,29. In contrast, video data on antennal SSRs are available for Drosophila, the navel orangeworm moth (Amyeloistransitella), Schistocerca Americana, and the bed bug (Cimex lectularius)16,30,31,32,33.

Locust palp sensilla basiconica have a particular structure that differs from that of locust antennal sensilla and many other insect sensilla. Using the method described here, action potentials generated by locust palp sensilla basiconica subtypes pb1 and pb2 could be recorded and discriminated (Figure 4 and Figure 5).

The critical step is the penetration of the recording electrode. The recording electrode should be inserted into the base of the sensillum and advanced until good signals are acquired. In addition, it is important to prevent the dome of the palp from collapsing when the recording electrode is inserted into the base of the sensillum. To achieve this, we set up a platform including a special locust maxillary palp holder (MPH) and used a tungsten wire to compress the fourth segment of the palp. Many repetitions of this procedure demonstrate that this is effective. Based on the response patterns of the neurons in the sensilla to several odorants, we have, for the first time, identified two subtypes of sensilla basiconica on the locust maxillary palp, namely pb1 and pb2.

The limitation of the technique outlined in this publication is that it could be used to record big insects (e.g., moths, beetles, and locusts) while not to record small insects (e.g., flies and mosquitoes), which have their own platforms and techniques13,14,15,16,17. This technique is complementary to existing methods.

In conclusion, a highly effective protocol of an SSR from insect palp sensilla basiconica is described in detail. This protocol could provide researchers with a useful technique in the study of molecular and cellular mechanisms of insect olfaction on the mouthpart. This method linked with gas chromatography could be used to identify natural electrophysiologically-active ligands in extracts of favorable food resources.

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The authors have nothing to disclose.


This work is supported by a grant from the National Natural Science Foundation of China (No.31472037). Any mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply a recommendation.


Name Company Catalog Number Comments
Tungsten wire ADVENT W559504 Used for making the electrode and fixing the palp
NaNO2 Sigma-aldrich 563218-25G Used for sharpening the tungsten wire
AC Power Supply Syntech A2-70 Providing the voltage in sharpening the tungsten wire
Stereoscope Motic SMZ-163 Used for observing the sharpening of tungsten wire
Microscope Olympus W-51 Used for observing the sensilla on locust maxillary palp
Intelligent Data Acquisition Controller Syntech IDAC-4 Real-time on screen display of all signals before and during recording
Stimulus controller Syntech CS-55 Used for controlling the stimulus application
Electronic micromanipulator C.M.D.T CFT-8301D Used for minor movement of the recording electrode
Micromanipulator Narishige MN-151 Used for minor movement of the reference electrode
Speaker EDIFIER R101T06 Connected with IDAC-4 and providing sound for the signal
Magnetic base PDOK PD-101 Used to hold the electrode, and stimulus delivery tube
Vibration Isolation Table TianHe HAP-100-1208 Used for isolating the vibration from the equipment
Glass slide CITOGLAS ZBP-407 Used for making the base for the MPH
Blu-tack Bostik Blu-tack-45g Fixing the tungsten wire
Pasteur tube YARE WITEG Placing the filter paper containing stimuli stimulus solutions



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Single Sensillum Recordings Locust Palp Sensilla Basiconica Chemoreception Field Odorant Receptor Tungsten Electrodes Sodium Nitrite Stimulus Solutions Mineral Oil 1-Nonanol Nonanoic Acid E-2-Hexenal Hexanal Pasteur Tubes Filter Paper Strips Locust Maxillary Palp Holder
Single Sensillum Recordings for Locust Palp Sensilla Basiconica
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Cite this Article

Li, H., You, Y., Zhang, L. SingleMore

Li, H., You, Y., Zhang, L. Single Sensillum Recordings for Locust Palp Sensilla Basiconica. J. Vis. Exp. (136), e57863, doi:10.3791/57863 (2018).

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