Sodium Taurocholate Induced Severe Acute Pancreatitis in C57BL/6 Mice

Summary

Animal models for severe acute pancreatitis enable the study of pathophysiological changes at the initial stage, facilitating observation of the evolution of inflammatory events. Here we provide a protocol for the induction of severe acute biliary pancreatitis by retrograde infusion of sodium taurocholate into the pancreatic duct of anesthetized C57BL/6 mice.

Abstract

Biliary acute pancreatitis induction by sodium taurocholate infusion has been widely used by the scientific community due to the representation of the human clinical condition and reproduction of inflammatory events corresponding to the onset of clinical biliary pancreatitis. The severity of pancreatic damage can be assessed by measuring the concentration, speed, and volume of the infused bile acid. This study provides an updated checklist of the materials and methods used in the protocol reproduction and shows the main results from this acute pancreatitis (AP) model. Most of the previous publications have limited themselves to reproducing this model in rats. We have applied this method in mice, which provides additional advantages (i.e., the availability of an arsenal of reagents and antibodies for these animals along with the possibility of working with genetically modified strains of mice) that may be relevant to the study. For acute pancreatitis induction in mice, we present a systematic protocol, with a defined dose of 2.5% sodium taurocholate at an infusion speed 10 µL/min for 3 min in C57BL/6 mice that reaches its maximal level of severity within 12 h of induction and highlight results with outcomes that validate the method. With practice and technique, the total estimated time, from the induction of anesthesia to the completion of the infusion, is 25 min per animal.

Introduction

In humans, the presence of gallstones is the most common cause of pancreatitis due to the obstruction of the terminal portion of the choledochal, interrupting the flow of pancreatic secretions and causing an intense inflammatory process in the pancreas, with an increase in the concentration of digestive enzymes in the serum and inflammatory mediators^1,2.

Two different theories have been proposed to explain the development of acute pancreatitis (AP). The "common channel" theory suggests that the stones present in the gallbladder obstruct the distal common bile duct system, allowing bile secretion to flow retrograde into the pancreatic duct. The second theory (the "duct obstruction" theory) suggests that the obstruction of the pancreatic duct by excess gallstones causes a blockage in the flow of pancreatic secretion to the duodenum, causing ductal hypertension³. Although the mechanisms that lead to acute biliary pancreatitis are not fully understood, the outcome is an intense inflammatory process. Digestive enzyme eruption and pancreas self-digestion lead to histopathological changes, an increase in inflammatory cytokines (IL-1β, IL-6, TNF-α) in ascitic fluid and serum, and an increase in acute phase proteins^4,5,6.

Severe acute pancreatitis is a condition that deserves clinical attention due to the involvement of multiple organs and a high mortality risk. Animal models for the reproduction of acute pancreatitis (AP) are important as these explain the pathophysiological mechanisms of the disease and help in monitoring the evolution of inflammatory events, starting from the initial stages of the disease. This is usually not possible in the clinics^2,7. In addition, access to pancreatic tissues is easy in preclinical studies, favoring the elucidation of changes linked to clinical conditions⁸ along with the possibility of working with isogenic species, eliminating undesirable variables, and mirroring clinical similarity with the outcomes observed in the human condition⁹.

Biliary and non-biliary models for the induction of acute pancreatitis in rats and mice species have been frequently studied in the scientific literature. Non-biliary methods of induction include administration of supramaximal stimulating doses of the cholecystokinin secretagogue or its analog cerulein¹⁰; administration of almost lethal doses of L-arginine; or administration of a choline-deficient diet supplemented with ethionine¹¹. Although these methods are easy to reproduce and result in pancreatic inflammation, they do not replicate the mechanisms that in theory trigger AP (i.e., the reflux of bile secretion into the pancreatic duct). The technique that addresses the biliary model is based on the retrograde infusion of bile acids into the pancreatic duct and requires well-trained researchers to carry out this protocol. Several studies have been published using this method in rats (apparently for technical reasons since these experiments involve surgical procedures)^12,13. However, the approach in mice may offer more interesting outcomes in the study of inflammation^3,14,15. In this study, we will show a checklist of the steps to be followed for the reproduction of severe acute pancreatitis by infusion of sodium taurocholate in C57BL/6 anesthetized mice.

For works that involve the need for experiments with antibodies and analysis of the gene and protein expression, the use of mice is preferable because of the greater arsenal of materials for these animals and the possibility of working with isogenic and knockout species, among others that can be used relevant to studies¹⁶. Mice C57BL/6 is an inbred strain of mice originally developed for the study of antitumor activity and immunology. This strain is increasingly being preferred by researchers for being isogenic, allowing for a greater reproducibility of results, which may imply the use of a smaller number of animals in an experiment and less variability of results between the same group^17,18.

Perides et al. (2010)¹⁴ published a protocol for AP induction in mice by sodium taurocholate infusion. Here we update this model using a higher sodium taurocholate concentration (2.5%) in C57BL/6 mice, with a defined volume and speed of infusion (Figure 1). The maximal level of severity is reached within 12 h of induction in mice. The elevation of the concentration of IL-6 both in the serum and in the peritoneal cavity is correlated with the progression of AP. With practice, the total estimated time from the induction of anesthesia to the completion of the infusion, is 25 min per animal. It is essential that a trained researcher conducts this experiment. To ensure that the solution is properly injected into the common bile duct, perform several pilot training sessions using methylene blue instead of sodium taurocholate.

Protocol

This protocol was approved by the Ethics Committee for the use of animals of the USP Medicine School, Num. Project: 1343/2019-CEUA: FMUSP. For this protocol, C57BL/6 mice, aged 6 weeks, weighing 20 ± 2 g were used (n = 9/group).

1. Laparotomy

1. Anesthetize animals with xylazine (10 mg/kg) and ketamine solution (80 mg/kg), subcutaneously (0.1 mL/10g of body weight) using a 1 mL syringe and 13x0.45mm needle 26G ½. Check for sufficient anesthesia depth by pinching the toe. Control body temperature using heated pads. Ensure that all surgical materials are sterile.
2. Clean the abdominal area with 5% povidone-iodine solution and use a trimmer to remove hair between the chest and lower abdomen (approximately 2 cm²). Clean the surgical area with 70% alcohol.
3. Immobilize the animal on the surgical board using surgical tape. Use scissors to cut 5 mm of the skin horizontally, on the upper part of the abdomen and 1 cm below the xiphoid process. Repeat the cut on the peritoneum. This will result in a laparotomy with minimal exposure of the cavity.

2. Locating and exposing the pancreas

1. With the aid of a retractor, pull the liver towards the mouse's head, ~1 cm from the intestine.
2. Locate the region of the pancreas that will be injected with sodium taurocholate (pancreas head). Locate the duodenum with reference to the liver- below the liver, on the right side (on the left as the mouse is viewed). The duodenum is the first part of the small intestine and is connected to the final portion of the stomach.
3. With the aid of forceps, lift the liver towards the animal head, and gently pull the small intestine portion. Fix the two lateral ends of the small intestine with a 6-0 polypropylene suture to better view the distal portion of the common bile duct.

3. Severe acute pancreatitis induction

1. Temporarily occlude the proximal common bile duct with a microvessel clip to prevent retrograde infusion from leaking into the liver. The common bile duct can be seen on the liver side of the duodenum and its junction with the duodenum will appear white. Expose the organ out of the abdominal cavity.
2. Puncture the periampullary region (whitish part of the small intestine's wall) to access the common bile duct with a 0.4 mm needle connected to a 0.54 mm polyethylene tube.
3. Make a temporary occlusion of the distal common bile duct with 8-0 suture to prevent the sodium taurocholate solution from leaking into the duodenum.
4. Start the infusion pump and program a 2.5% sodium taurocholate solution (diluted in 0.9% saline) infusion at a constant speed of 10 µL/10 g body weight for 3 min.
5. After the infusion, remove the microvessel clip, the temporary 8-0 suture, and the injection needle from the bile pancreatic duct to reconstitute the physiological flow of the bile.
6. At the end, suture the abdomen with 6-0 nonabsorbent monofilament polypropylene suture. The time between the laparotomy and the end suture should be a maximum of 30 min (see Figure 1).
7. After the surgery, house the animals in polyethylene boxes lined with wood shavings and water and food ad libitum.
8. Treat control mice in the same manner as the experimental mice but ensure that the infusate consists of saline only. Perform the surgical procedure and the infusion of saline solution (10 mL/min, for 3 min) in a control group (SHAM) to eliminate the inflammatory bias caused by surgery and cannulation.
9. Use tramadol 12.5 mg/kg subcutaneously every 8 hours, starting after post-surgical recovery.

4. Methods for analysis

1. At 12 h after AP induction, anesthetize the animals with xylazine (10 mg/kg) and ketamine (80 mg/kg) to collect approximately 250 µL of blood via the orbital plexus.
   1. Gently hold the skin on the back, promoting a slight protrusion of the eyeball, and position it with the eye facing upwards.
   2. Instill a drop of eye ointment containing local anesthetic in the animal's eye.
   3. Position the end of the capillary tube in the medial corner of the eye and insert it gently under the eyeball, with an angle of ~30°-45°. Rotate the capillary tube until blood flow begins. Remember that it is not necessary to use force for the procedure.
   4. Once the collection is over, ensure homeostasis by keeping the eyelids closed by light compression with the gauze. Discard the capillary tube in the sharps container¹⁹.
   5. Centrifuge the serum (700 x g, 15 min) and stock the supernatant for amylase and IL-6 dosing (step 4.7 and 4.8).
2. Euthanize mice by CO₂ asphyxiation.
3. Use a 27 G needle to inject 4 mL of ice-cold 1x PBS into the peritoneal cavity. Tened the abdominal skin and ensure that the needle is pushed slowly in the peritoneum to not puncture any organs. After the injection, gently massage the peritoneum for 10 s to remove cells adhered to the peritoneum.
4. Using scissors and tweezers, make a small cut (0.5 cm) on the inner skin and musculature to expose the abdominal cavity. Insert a bulb pipette in the peritoneum and collect the fluid. Be careful not to aspirate fatty tissue or other organs.
5. Collect as much fluid as possible and deposit the collected cell suspension in tubes kept on ice. Discard the bulb pipette in the sharps container²⁰. Centrifuge the peritoneal fluid (250 x g, 5 min) and stock the supernatant for IL-6 dosing (step 4.9).
6. Collect the pancreas region adjacent to the duodenum (<5mm).
7. Process the pancreas by fixing in 10% formalin and incorporate it into paraffin.
   1. Stain the slides with hematoxylin and eosin for histopathological analyzes under light microscopy. Use Schmidt's protocol²¹ (pancreatic edema, acinar cell, injury/necrosis, pancreatic inflammation) to assess the extent of AP.
8. Measure amylase (U/dL) using commercially available kits according to the manufacturer's recommendations.
9. Measure IL-6 by Luminex assays using commercial kits according to the manufacturer's recommendations.
10. Store the serum and peritoneal fluid supernatant obtained in the steps 4.1 and 4.4 in a freezer at -80 °C if needed.

Results

Pancreatitis severity was scored between 0-3 according to the Schmidt's scale²¹ where zero corresponds to the absence, 1 corresponds to a mild presence (<25%), 2 corresponds to a moderate presence (between 25 and 50%) and 3 corresponds to an intense presence (> 50%) (Table 1). The measurements performed were plasma amylase activity, pancreatic edema, acinar cell, injury/necrosis, pancreatic inflammation (by histology analysis of H&E-stain...

Discussion

The method of inducing acute pancreatitis by retrograde sodium taurocholate infusion has already been shown in rats^22,23,24. Three similar works, published in 2008, 2010 and 2015, served as a reference for the protocol^3,14,15. In this work, we list all the critical steps for reproducing this method in C57BL/6 mice and some possibilities...

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
0.4 mm needle	INTRAG MEDICAL TECH	90183210	30G
0.54 mm polyethylene tube	Tygon	730010	-
Styrofoam block	-	-	-
masking tape for mounting the mouse	Missner	1236	-
Infusion pump scheduled to 10µL / min.	Havard aparatus-Peristaltic Pump Series	MA1 55-7766	Model 66 Small Peristaltic
Scissors and forceps
Antiseptic providine iodine	Pfizer	12086OR	antisepsis
70% ethanol	SIGMA	459836	Mix 700 mL 100% ethanol with 300 mL dH2O
Razor blade	Lord	bdk9a1ghk6	For trichotomy
Sodium taurocholate	Sigma-Aldrich	86339- 1G	CAS NUMBER- 345909-26-4
microvessel clip	Medicon Surgical	56.87.35	Approximator, opening 4.0 mm, closing pressure 30 - 40 g
6-0 prolene	Bioline	5162	Suture line
Ketamin NP (cloridrato de dextrocetamina) 50mg/mL	Cristália
Xilazine 2%	Syntec
Sterile saline solution (0.9% (wt/vol) saline)	Farmace	105851
Methyl Blue	Sigma-Aldrich Chemicals	M5528
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay	MERCK	MCYTOMAG-70K	Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Amylase Assay	Labtest	11
Desmarres retractor 13-mm width	ROBOZ	RS-6672