Erratum: Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research

Summary

An erratum was issued for: Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research. The Abstract and Representative Results sections were updated.

Abstract

This corrects the article 10.3791/68941

Protocol

An erratum was issued for: Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research. The Abstract and Representative Results sections were updated.

The second to last sentence of the Abstract was updated from:

Representative results from healthy embryos demonstrated consistent ventricular contraction and robust functional indices, while embryos exposed to cadmium showed impaired cardiac morphology and significantly reduced cardiac output.

to:

Representative results from healthy embryos demonstrated consistent ventricular contraction and robust functional indices, while embryos exposed to cadmium showed impaired cardiac morphology and significantly increased cardiac output.

The fifth paragraph of the Representative Results was updated from:

Figure 5 shows data from embryos with suboptimal developmental morphology due to cadmium exposure during incubation. In these embryos, cardiac morphology was often altered, with enlarged or misshapen ventricles, diminished contractility, and the frequent occurrence of pericardial edema. In such cases, end-systolic and end-diastolic volumes were closer in value, leading to lower stroke volumes (SV = 0.497 nL± 0.2 nL). Heart rate was also significantly reduced (130 bpm ± 7 bpm), resulting in lower cardiac output (CO = 56.1 nL/min ± 24.8 nL/min). These results demonstrate the sensitivity of this method in detecting early functional impairment. Overall, these results confirm the robustness and sensitivity of the method in assessing cardiac morphology and function during early zebrafish development. The ability to visualize and quantify dynamic cardiac parameters enables reliable differentiation between normal and aberrant development, even in the absence of overt structural malformations.

to:

Figure 5 shows data from embryos with suboptimal developmental morphology due to cadmium exposure during incubation. In these embryos, cardiac morphology was often altered, with enlarged or misshapen ventricles, diminished contractility, and the frequent occurrence of pericardial edema. In such cases, end-systolic and end-diastolic volumes were closer in value, leading to higher stroke volumes (SV = 0.497 nL± 0.2 nL). Heart rate was significantly reduced (130 bpm ± 7 bpm), resulting in higher cardiac output (CO = 56.1 nL/min ± 24.8 nL/min). These results demonstrate the sensitivity of this method in detecting early functional impairment. Overall, these results confirm the robustness and sensitivity of the method in assessing cardiac morphology and function during early zebrafish development. The ability to visualize and quantify dynamic cardiac parameters enables reliable differentiation between normal and aberrant development, even in the absence of overt structural malformations.

The legend for Figure 5 was updated from:

Figure 5: Functional cardiac impairment in zebrafish embryos exposed to cadmium. Representative data from embryos exhibiting abnormal cardiac development following cadmium exposure. Morphological changes include ventricular dilation and pericardial edema (A). Functional measurements revealed: (B) lower SV (0.497 ± 0.2 nL), (C) reduced heart rate (130 bpm ± 7 bpm), and (D) diminished CO (56.1 nL/min ± 24.8 nL/min), demonstrating the assay's sensitivity to toxic insult. Scale bar: 100 µm. 

to:

Figure 5: Functional cardiac impairment in zebrafish embryos exposed to cadmium. Representative data from embryos exhibiting abnormal cardiac development following cadmium exposure. Morphological changes include ventricular dilation and pericardial edema (A). Functional measurements revealed: (B) higher SV (0.497 ± 0.2 nL), (C) increased CO (56.1 nL/min ± 24.8 nL/min), and (D) reduced heart rate (130 bpm ± 7 bpm), demonstrating the assay's sensitivity to toxic insult. Scale bar: 100 µm.