We describe a physical constriction method to make mini embryos from Xenopus laevis 1-cell embryos.
Method Article
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 30-G needle | BD Medical | 305128 | For puncturing embryos |
| 60 mm Disposable Petri Dishes | VWR | 25384-092 | For incubating embryos |
| ACCU-SCOPE 3075 Zoom Stereomicroscopes | VWR | 470351-130 | For visualizing embryos |
| Agarose | Sigma-Aldrich | A-9414 | For coating petri dishes |
| Dumont Tweezer, Style 5 | Electron Microscopy Sciences | 72701-D | For tweaking and holding embryos |
| Ficoll 400 | Sigma-Aldrich | GE17-0300-10 | For incubating embryos |
| TipOne Pipette Tip, 10 µL | USA Scientific | 1111-3700 | For mounting the hair knots |
| Wax Block, Black | Carolina Biological Supply Co. | 974228 | For mounting the hair knots |
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