Stereo Microscopy
Stereo Microscopy - Related Videos
10:22
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
0 Views •
2025
This article presents a protocol to perform comprehensive spatial transcriptomic profiling of host, viral, fungal, and microbiome RNA from formalin-fixed paraffin-embedded tissue sections using Stereo-seq, which enables high-resolution mapping of diverse transcriptomes while preserving tissue architecture.
09:32
Development of New Methods for Quantifying Fish Density Using Underwater Stereo-video Tools
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Cited by 9 •
2017
We describe a new method for counting fishes, and estimating relative abundance (MaxN) and fish density using rotating stereo-video camera systems. We also demonstrate how to use distance from camera (Z distance) to estimate species-specific detectability.
14:14
Quantification of Strain in a Porcine Model of Skin Expansion Using Multi-View Stereo and Isogeometric Kinematics
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Cited by 9 •
2017
This protocol uses multi-view stereo to generate three-dimensional (3D) models out of uncalibrated sequences of photographs, making it affordable and adjustable to a surgical setting. Strain maps between the 3D models are quantified with spline-based isogeometric kinematics, which facilitate representation of smooth surfaces over coarse meshes sharing the same parameterization.
05:54
Stereo-Electro-Encephalo-Graphy (SEEG) With Robotic Assistance in the Presurgical Evaluation of Medical Refractory Epilepsy: A Technical Note
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Cited by 20 •
2016
Stereo-electroencephalography (SEEG) aids in localization of epileptogenic zones, however, remains relatively underutilized in the United States. The goal of this abstract is to provide a brief introduction to the technique of SEEG and further a detailed technique of using robotic assistance in the placement of SEEG electrodes.
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 105 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
FAQs
Depth perception in Stereo microscopy comes from presenting slightly different views through separate optical paths. This visual difference allows observers to judge relative position rather than seeing only a flat image. The resulting spatial information helps when biological structures overlap or when researchers need to evaluate how parts of a specimen are arranged in relation to one another.
Stereo microscopy can use reflected or transmitted illumination, allowing researchers to examine different aspects of biological specimens. Reflected light supports observation of surfaces, while transmitted light can assist with viewing specimens through the illuminated field. Having both approaches available makes the technique adaptable to organisms, tissues, developmental stages, and other samples requiring different viewing conditions.
The large working distance leaves space between the objective and the specimen, so researchers can manipulate the sample while viewing it. This feature is important for tasks such as dissection and microinjection, where access to the specimen must be maintained during observation. It also distinguishes the technique from microscopy focused primarily on high-resolution imaging.
Stereo microscopy emphasizes three-dimensional viewing, low magnification, and practical access to the specimen, whereas compound microscopy is associated here with higher-resolution imaging. The two approaches therefore serve different needs: stereo observation supports handling, sorting, and spatial assessment, while compound microscopy is more appropriate when fine detail at higher magnification is the primary goal.
A biological workflow may begin with placing a specimen for direct observation, followed by sorting, dissection, or microinjection while the image remains visible. Extensive preparation is often unnecessary, which helps researchers move efficiently from examination to manipulation. The same setup can support observation before, during, and after these practical steps.
Researchers can apply Stereo microscopy to organisms, tissues, surfaces, and developmental stages when they need a spatially informative view without extensive specimen preparation. It is particularly useful for sorting samples, examining visible structures, and carrying out manipulations under observation. These uses make the technique relevant to routine biological handling as well as developmental studies.
Stereo microscopy provides more spatial information and magnification than unaided observation while preserving enough working space for specimen handling. At the same time, it does not replace high-resolution compound microscopy when very fine detail is required. In biology, this intermediate role makes it useful for selecting, positioning, and manipulating specimens before more detailed imaging.