Guest Editor
Collection Overview
Coherent Raman scattering microscopy (CRM) visualizes molecules’ distribution with high spatial (<1 µm) and temporal resolution (~1 sec) in intact biological samples based on their intrinsic vibrational contrasts. The number of applications using CRM has increased significantly over the last two decades. There is a surge of interest in applying CRM to study lipid metabolism, cancer diagnosis, neurodegeneration, and drug transport. However, the technical barrier remains high for non-experts looking to use a coherent Raman microscope for bioimaging. Unlike commercial confocal fluorescence microscopes, many coherent Raman microscopes used to date still require custom configurations of lasers, microscopes, and detection systems. There are also no established standards in determining imaging performance. This collection of CRM methods aims to provide detailed guidance on best practices in using two major types of CRM – coherent anti-Stokes Raman scattering (CARS) microscopy and stimulated Raman scattering (SRS) microscopy.
Editorial
Articles
In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
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Cited by 5
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2021
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1Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology, 2State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science and Technology, 3Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, 4Molecular Neuroscience Center, The Hong Kong University of Science and Technology, 5Department of Biology, School of Life Sciences, Southern University of Science and Technology
