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Current Methods in Gonad Research

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Kai Xia

Kai Xia

Sun Yat-sen University, Zhongshan School of Medicine

Dr. Kai Xia studied clinical medicine as an undergraduate and then completed his PhD from the Department of Urology, at the First Affiliated Hospital, Sun Yat-sen University. During his PhD training, he studied the mechanisms behind testosterone deficiency and the stem cell-based therapy that could recover testosterone production. Kai then joined the lab of Prof. Andy Peng Xiang as a postdoctoral researcher at the Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Guangzhou, China. His current research focuses on the development and aging of gonad with the help of mouse transgenic mice, single-cell sequencing, and AAV-mediated gene delivery.

Collection Overview

Gonads are vital organs in reproductive biology and endocrinology, responsible for gamete production, hormone synthesis, and the regulation of reproductive function. Research on gonads is essential for advancing our understanding of fertility, reproductive disorders, endocrine signaling, and developmental biology. With growing global concerns about declining fertility rates and reproductive health challenges, there is an urgent need to share innovative and reproducible methods that can drive progress in this field.


The study of gonads presents unique challenges due to their complex tissue architecture, dynamic cellular interactions, and sensitivity to environmental and genetic factors. Researchers often face difficulties in developing models that accurately mimic in vivo processes, capturing high-resolution structural and functional data, and standardizing approaches for cross-species studies. These hurdles underscore the importance of comprehensive methodological resources to support diverse research needs.


This collection aims to bring together a wide range of current and emerging techniques used in gonad research. By showcasing methodological advances in JoVE’s visual format, it will provide researchers with the tools to refine experimental approaches, enhance reproducibility, and foster collaboration across disciplines. This resource will significantly benefit the scientific community by enabling breakthroughs in reproductive health, developmental biology, and translational applications.

Articles

CRISPR/Cas9-mediated Endogenous Fluorescent Tagging of Germline-specific Genes in <em>Caenorhabditis elegans</em>
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CRISPR/Cas9-mediated Endogenous Fluorescent Tagging of Germline-specific Genes in Caenorhabditis elegans

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2026

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