Collection-image

TOPICAL COLLECTIONS

Experimental Methods and Techniques in Chlamydomonas reinhardtii

Submit Abstract

Guest Editors

Amy Ikui

Amy Ikui

Brooklyn College-The City University of New York

Ikui’s lab studies how DNA replication and cell division are coordinated in cell cycle. They use single-celled model organisms, Saccharomyces cerevisiae and Chlamydomonas reinhardtii, to understand how eukaryotic cells maintain genome integrity throughout the cell division. http://amyikuilab.com

Masayuki Onishi

Masayuki Onishi

Duke University, Department of Biology

Masayuki Onishi graduated from the University of Tokyo with a thesis on the role of septin-family proteins during sporulation in fission yeast Schizosaccharomyces pombe. He then received a postdoc training at Stanford University Department of Genetics under supervision of Dr. John R. Pringle studying the mechanisms of cell division in budding yeast Saccharomyces cerevisiae and the green alga Chlamydomonas reinhardtii. At Duke, he runs a research lab that studies the mechanisms of cell division, cell cycle, organellar distribution, and the cytoskeleton. The Onishi lab is interested in the evolution of the mechanisms of cell division in eukaryotes. Currently, the lab uses the green alga Chlamydomonas reinhardtii as a model and investigates the roles of three elements of the cytoskeleton, F-actin, microtubules, and septin.

Collection Overview

Chlamydomonas reinhardtii is a single-celled green algae used as a model organism for both plants and animals to study various cellular functions. The objective of this collection is to share standard experimental procedures and methods such as strain maintenance, share genetic cross method, introduce time-lapse microscopy set up, share gene editing protocols, introduce this model organism to other communities, and create a new networking platform for the Chlamydomonas community. We believe that the information in the collection will accelerate our research by sharing methods via online video format, which will globally encourage scientists to continue or start using Chlamydomonas as a model system.

Articles

Reactivation of Demembranated Cell Models in <em>Chlamydomonas reinhardtii</em>
3:37

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

0 Views

•

Cited by 1

•

2022

•