Inflammation is an essential physiological response of the organism which aims to restore homeostasis after infection or tissue injury. The main cells involved in the inflammatory process are neutrophils, the most abundant leukocyte in the circulation. Neutrophils circulate in a steady state until be recruited to a site of infection or injury, where they execute a vast array of specialized functions. Once transmigrated through blood vessels, neutrophils recognize molecular patterns present in microorganisms or damaged cells to be activated and initialize effector activities. Neutrophils act by three main antimicrobial functions: phagocytosis, degranulation, and the release of nuclear material in the form of neutrophil extracellular traps (NETs). With a short lifespan, after activation, neutrophils undergo apoptosis and should be removed. The removal of neutrophils is executed by macrophage and dendritic cells in a process called efferocytosis. This process is important to avoid tissue damage due to the powerful enzymatic activity of neutrophils and to restore tissue homeostasis. Different methodologies have been employed to access neutrophil activities and to help better understand the ambiguity and complexity of the inflammation process. This Methods Collection intends to gather both, established and novel techniques to assay neutrophil tasks from circulation until its death and removal, to inspire researchers and shed light on approaches of inflammation study.