Glaucoma is a degenerative disease that culminates in blindness. It is pathologically characterized by a loss of retinal ganglion cells (RGCs) and their axons from various aetiologies. In spite of many significant advances in our knowledge and the clinical management of glaucoma, the pathological processes underlying its different aetiologies are unclear and existing treatments are not always effective. Pre-clinical models are invaluable for appreciating the underlying pathological mechanisms and developing appropriate translational strategies. The aim of this Methods Collection is to collate existing and emerging pre-clinical models of glaucoma into a compendium for scientists working in, as well as those newly entering the field. The goal is to enable researchers to readily compare and assess the merits and limitations of the various models along with the technical difficulties in implementing them. We invite researchers to submit articles detailing their model and the clinical features it replicates.