Guest Editors
José Teixeira
University of Coimbra, CNC - Center for Neuroscience and Cell Biology, CIBB - Center for Innovative Biomedicine and Biotechnology, Coimbra, Portugal
José Teixeira graduated in Biochemistry from the University of Trás-os-Montes and Alto Douro in Portugal (2009) and obtained the MSc in Biochemistry from the University of Porto in Portugal (2011) and is completing his PhD in Pharmaceutical Sciences. José Teixeira is a Junior PhD Researcher in the MitoXT – Mitochondrial Toxicology and Experimental Therapeutics group at the Center for Neuroscience and Cell Biology (CNC), University of Coimbra (UC), Portugal. Dr. Teixeira research interests are focused on the role of mitochondria in oxidative stress-related pathologies and in the development and evaluation of the potential biological effects of mitochondria-targeted antioxidants based on naturally dietary scaffolds as a therapeutic solution. Particularly in debilitating diseases that have little or no effective treatments with an unmet need for efficient therapeutics, notably Non-Alcoholic Fatty Liver Disease (NAFLD).
João Pessoa
University of Coimbra, CNC - Center for Neuroscience and Cell Biology, CIBB - Center for Innovative Biomedicine and Biotechnology, Coimbra, Portugal
Dr. João Pessoa is a Postdoctoral Researcher in metabolism at the University of Coimbra, Center for Neuroscience and Cell Biology (Portugal). He completed his PhD in Biomedical Sciences at the University of Porto (Portugal) in 2014. He did his first postdoctoral stage in gene expression at the University of Lisbon (Portugal), which included a secondment at the University of Buenos Aires (Argentina). In 2021, he relocated to the University of Coimbra. He seeks understanding how proteins defining liver cell morphology contribute to the pathophysiology of Non-Alcoholic Fatty Liver Disease (NAFLD), to ultimately exploit those proteins as diagnosis or treatment targets. Dr. Pessoa was a selected speaker at the 18th FEBS Young Scientists’ Forum, is a member of the Marie Curie Alumni Association (MCAA), was interviewed for the BioPhotonics magazine and is a Guest Associate Editor for Frontiers in Oncology.
Collection Overview
Non-Alcoholic Fatty Liver Disease (NAFLD) is characterized by fat accumulation in more than 5% of hepatocytes and affects about 25% of the global adult population. In its initial stage, nonalcoholic fatty liver (NAFL), fat accumulation has no apparent consequences in hepatic function. However, its evolution into non-alcoholic steatohepatitis (NASH) correlates with extensive inflammation and fibrosis. NAFLD is a major cause of cirrhosis and liver cancer and an overwhelming burden to healthcare. Accurate diagnosis and staging of NAFLD is critical for differentiating between reversible NAFL and irreversible NASH. The current gold standard is histological analysis of a liver biopsy; however, the risks associated with this invasive procedure urge the development of alternative diagnosis approaches. Current NAFLD treatments are largely based on diet and lifestyle alterations; however, these interventions have limited effectiveness, especially in lean patients. Therefore, alternative treatment approaches are also urgently needed. These have to be tested in cellular and animal models, followed by clinical trials, before becoming accessible to patients. A common link in the development of these novel approaches is method testing and validation. In this collection, we encourage submission of abstracts describing methods in NAFLD context, with a special focus on novel approaches for in vitro and in vivo diagnosis or treatment. We aim at providing a source of easy-to-reproduce protocols for researchers in NAFLD.
Articles
Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment
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Cited by 6
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2023
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Karina Garcia1,2,3,4, André Alves1,2,3, Teresa M. Ribeiro-Rodrigues1,2,3, Flávio Reis1,2,3, Sofia Viana1,2,3,4
1Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, 2Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 3Clinical Academic Center of Coimbra (CACC), 4Polytechnic Institute of Coimbra, Coimbra Health School
Abstracts
Scalable 3D printed inserts for increased stability of hepatic intravital microscopy
Natalie Porat-Shliom*1,
Mercedes Cornelius-Muwanguzi 1
1Thoracic and GI Malignancies Branch, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD.