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TOPICAL COLLECTIONS

Current Methods in Therapeutic Applications of Pluripotent Stem Cells: Derivation, Differentiation, and Characterization

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Guest Editors

Mourad A. M. Aboul-Soud

Mourad A. M. Aboul-Soud

King Saud University, Riyadh 11433, Saudi Arabia, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Chair of Medical and Molecular Genetics Research

Mourad A. M. Aboul-Soud research interests are multidisciplinary covering diverse microbial, mammalian human, and plant biological systems. His main research tracks include pharmacological activities of natural products, clinical biochemistry, biochemistry, molecular biology, genetic engineering, and environmental toxicology. In this context, his solid track record as a researcher is reflected in the publication of 60 high-quality peer-reviewed Clarivate Analytics articles in high-profile international journals (Source: Google Scholar: https://scholar.google.com/citations?hl=ar&user=ecUji3YAAAAJ&view_op=list_works&alert_preview_top_rm=2). His total number of citations in the Google Scholar database is 1313 and growing, with maximum citations per single article being 173, h-index of 20 (Clarivate Analytics h-index = 17) and i10-index 30. The current stem cell research in Prof. Aboul-Soud’s lab is funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology (KACST), Kingdom of Saudi Arabia, Award Number (14-MED690-02).

Moustapha Kassem

Moustapha Kassem

University of Southern Denmark, Odense, Denmark, Odense University Hospital and Institute of Clinical Research, Department of Endocrinology and Metabolism, Molecular Endocrinology and Stem Cell Research Unit

Moustapha Kassem is a Scientific Director of Stem Cell Treatment Centre, University Hospital of Odense, Odense, Denmark. He is a Principal investigator of the Indo-Danish Initiative of Stem Cells and Nanotechnology which is a collaboration between Universities of Southern Denmark, University of Aarhus, and Stem Cell Centre at Bangalore and Kerala, India. He is a Regular reviewer for Nature Medicine, Nature Biotechnology, Journal of Bone and Mineral Research, Bone, European Journal of Clinical Investigation, Endocrinology, European Journal of Endocrinology, International Journal of Developmental Biology, Calcified Tissue International, Stem Cells, Tissue Engineering, European Journal of Hematology, Biogerontology, Osteoporosis International. He has also reviewed grants for Medical Research councils in Norway, the UK, Austria, Singapore, China, Finland, and France. He reviewed PhD thesis and Dr. Med’s (doctorate degree of Medical Sciences) thesis. He has an Evaluation of professorships. He has lectured frequently at national and international meetings. In 2015 and 2020 more than 100 presentations at national and international meetings with plenary lectures, symposia presentation, meet the professor sessions in major stem cell and endocrinology conferences including the Annual Meeting of Endocrine Society, Annual meetings of American Society for Bone and Mineral Research, European Calcified Tissue Society and Annual meetings of European Society of Endocrinology.

Amer Mahmood

Amer Mahmood

King Saud University, Riyadh 11461, Saudi Arabia, College of Medicine, Department of Anatomy, Stem Cell Unit

Dr. Amer Mahmood received his MSc from Copenhagen University in the field of autoimmune destruction of b-cells in Type 1 diabetic patients. He obtained his PhD in Medical Sciences in the field of human Embryonic Pluripotent Stem Cell research and bone biology from the University of Southern Denmark. Where he successfully differentiated human embryonic stem cells into mesoderm and further in bone, fat, and cartilage forming mesenchymal stem cells. He did his post-doctoral fellowship at the Biomedical Research Center, Department of The Molecular Endocrinology & Stem Cell Research Unit (KMEB, University of Southern Denmark), where he studied the expression of human DLK-1 a novel regulator of bone mass that mediates estrogen deficiency-induced bone loss in mice. Dr. Amer was Assistant Professor and Principal Investigator in Stem cell research at King Saud University in the Stem Cell Unit Department of Anatomy. In 2017, his recent research focus on the field of Mesenchymal Stem Cells and Regenerative Medicine, particularly in skeletal-bone disorders. Dr. Amer has also been working extensively in the field of small RNA including micro-RNA and siRNA in addition to a Nano-fibrous scaffold for bone-, blood vessel, and cartilage regeneration. Also, in one of his projects, his group is looking at the differentiation of iPSCs into different mesodermal lineages. Dr. Amer’s group has also initiated the first clinical trial with adult stem cells in Saudi Arabia.  

Collection Overview

Stem cells possess unique characteristics of pluripotency and self-renewal abilities. Theoretically, human pluripotent stem cells (hPSCs) have the potential to be programmed to form any type of differentiated cells in the human body. Coupled with improvements in reprogramming techniques, this technology has advanced our knowledge of disease pathology and has accelerated the development of precise therapeutics. The inherent differentiation capacity into cell types that are derived from all the three possible germ layers renders hPSCs amenable for the treatment of various diseases and injuries. These favorable features and potentials presented hPSCs as a perfect and useful tool for disease modeling and regenerative medicine, whereby creating unprecedented opportunities and fueling advances in therapeutic clinical applications. The differentiation of three kinds of hPSCs, namely: mesenchymal stem cells (MSCs), embryonic stem cells (hESCs), and induced pluripotent stem cells (hiPSCs), into specific cell types has been largely employed as a promising model for investigating their potential therapeutic applications for diseases of human and drug discovery. The current methods collection focuses on some of the recent advances achieved in the generation, differentiation, characterization, and therapeutic applications of hPSCs.

Articles

Modeling Human Cerebellar Development <em>In Vitro</em> in 2D Structure
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Modeling Human Cerebellar Development In Vitro in 2D Structure

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Cited by 5

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2022

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Abstracts

Stepwise Generation and Validation of Midbrain Dopamine Neurons from Human Pluripotent Stem Cell

Tae Wan Kim*1

1Department of New Biology and 4Department of Biomedical Science and Engineering, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, South Korea