Guest Editors
Zulin Yu
Stowers Institute for Medical Research
After graduating from Nankai University with a BS in Physics, Yu earned his PhD studying acoustic communication in noise at Institute of Biophysics, Chinese Academy of Science. He joined the Stowers Microscopy team in 2009 as a specialist and then was named manager of Microscopy in 2021. Yu is an expert in super resolution microscopy techniques, including SIM, STED and STORM. Currently he is interested in developing and utilizing microscopy technologies to facilitate basic research at Stowers. He has published more than 40 scientific journals including Cell, Nature, eLife, P.N.A.S. et al.
Fengyan Deng
University of Kansas Medical Center, Department of Molecular and Integrative Physiology
Dr. Fengyan Deng received her PhD in Biochemistry and Molecular Biology in 2015 from Northwest A&F University, China. After that, she worked as a Research Associate at The University of Kansas Medical Center. Dr. Deng is a specialist in molecular biology, cellular biology, and extracellular vesicles (EVs) research and has extensive expertise in the fields of histology, immunology, and biochemistry. Her research focuses on the potential therapeutic role of different treatments induced EVs on Alzheimer’s disease (AD). AD characterized by declining memory and social performance and is acknowledged as the most commonly occurring neurodegenerative disease in the elderly. There is no cure or even a reliable treatment for AD as of now. A lifestyle intervention, such as exercise and heat treatment, can improve cognitive functions in AD patients. However, the mechanisms that mediate this change remain unknown. Increasing evidence reveals that heat shock proteins (HSPs) play important roles in AD progression by preventing protein misfolding and promoting refolding. Also lacking the consensus secretory signals, HSPs can be secreted into the extracellular environment through the EVs. Heat treatment and exercise, which increase the accumulation of HSPs in both cytosol and blood-derived EVs, have been used historically for their presumed therapeutic benefits. It is hypothesized that HSPs enriched EVs from healthy cells can get transferred to the extracellular milieu of the brain or even the brain cells, and further facilitate brain cells protection. Dr. Deng hopes to develop effective EV-based therapeutic strategies for AD.
Collection Overview
Histology has been the gold standard for studying the tissues of the body and how these tissues are arranged to constitute organs for centuries. Many histological methods have been developed to characterize tissue structures, understand disease progression and their causes at the molecular level. Traditional methods include Hematoxylin and Eosin staining, Masson's Trichrome staining, Masson's Trichrome staining, and so on for understanding the general structure of the tissue, Sirius Red staining to study collagen networks, Sudan staining to detect fat/lipids, Immunohistochemistry/Immunocytochemistry/Immunofluorescence for special protein staining, in situ hybridization/ Fluorescence in situ hybridization for visualization of special DNA/RNA. These methods have maintained their remarkable place in the histological and histopathological research field. However, these methods have their limitations: two-dimensional and low efficiency. Recently, several techniques including tissue clearing techniques, spatial genomics techniques, multiplex immunofluorescence have been developed to overcome these challenges. These advanced histological techniques will dramatically facilitate different biological research fields including genome-wide spatial gene expression and identification of stem-cell niches.
This Methods Collection describes a set of critically important and complementary histological techniques that may be used for different research models and different research fields.
Articles
Detection of Abnormal Prion Protein by Immunohistochemistry
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2023
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Leonor Orge1,2, Maria de Lurdes Pinto1, Paula Cristovão2, Paula Mendonça2, Paulo Carvalho2, Carla Lima3, Helena Santos2, Anabela Alves1, Fernanda Seixas1, Isabel Pires1, Adelina Gama1, Maria dos Anjos Pires1
1Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Science-AL4AnimalS, University of Trás-os-Montes and Alto Douro (UTAD), 2Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Oeiras, 3Pathology Laboratory, UEISPSA, National Institute for Agricultural and Veterinary Research (INIAV), Vairão