Vector species play a crucial role in the life cycle and transmission of various parasitic, bacterial, and viral diseases. We are only beginning to explore the direct impact these vectors have on influencing the immune response and, consequently, the disease outcomes in infected humans. One of the most significant challenges has been establishing viable colonies of relevant species; however, considerable progress has been made in this area, with viable colonies now achieved for several key species.

This Methods Collection aims to showcase a range of methodologies to study insect vectors of disease, including but not limited to, full life cycle studies of parasites, direct transmission studies, and the immunological insights these approaches provide.

This Collection will benefit the broader scientific community by presenting diverse methodologies, particularly in the fields of parasitology and vector-borne diseases. It will also highlight how these methods have opened new avenues of research, enhancing our understanding of host-vector-pathogen interactions and their roles in disease processes.