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Techniques for using “non-standard” animals for studying the neuronal basis of behaviors.

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William Kristan

William Kristan

University of California, San Diego

Dr. Kristan is a Distinguished Professor of Neurobiology at the University of California, San Diego. He received his PhD in Physiology at the University of Pennsylvania in 1969, working with Dr. George Gerstein on correlations among activity patterns of neurons in the sea slug, Aplysia. He then did a postdoctoral fellowship with Dr. Donald Kennedy at Stanford University, studying control of behaviors in the crayfish. From there he joined the laboratory of Dr. Gunther Stent as a Research Biologist at UC Berkeley, initiating studies on the neuronal basis of swimming in the medicinal leech. Dr. Kristan joined the Biology Department at UC San Diego in 1975 and spent his whole career at UCSD. In addition to his continued studies on leech swimming, he also studied the neuronal circuits for several other leech behaviors (bending, shortening, crawling, feeding) as well as the ways that these behaviors are chosen when activated simultaneously. Along with Dr. Kathleen French, he also characterized the development of leech neurons and their interconnections. His laboratory was among the first to use voltage-sensitive dyes to characterize a neuronal system, showing how leech systems produce the behaviors he studied and how leeched decide which behavior to produce in response to tactile stimuli. Since becoming Emeritus in 2016, Dr. Kristan has expanded his research interests to behavioral control in other leech species and in a planarian flatworm.  

Collection Overview

Characterizing neuronal circuits that produce behaviors is a major focus of neuroscience research, and the past two decades have seen a flowering of molecular, imaging, and computational techniques which facilitate such studies. Understandably, the development of these techniques has focused on a small number of animal species in which to work out technical details. However, with these techniques now in hand, we can ask which behaviors and which animals are best suited for such studies. Invertebrate animals (with their relatively small and accessible nervous systems) have contributed greatly to our understanding of this circuit/behavior interface, and the new techniques make it possible to study how systems of neurons generate behaviors. Once again, invertebrates can serve as productive living circuit boards revealing insights into how neuronal systems work. Due to the fact that molecular, imaging, and computational tools are now available for essentially any animal species, experimenters are no longer limited to using (for instance) only genetically tractable organisms. This methods collection presents the nervous systems of a number of invertebrates which are ripe for using the new techniques. Each video presents one or more animals, shows the dissection for exposing its nervous system, and gives an example of the data that can be generated from each animal. These techniques could also be used to characterize behaviors of many other species, maximizing the probability that any systems capability discovered is a general principle and not a peculiarity of a particular animal--a great strength of the comparative approach in biology.

Articles

Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing
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Planarian Scrunching as a Quantitative Behavioral Readout for Noxious Stimuli Sensing

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Cited by 9

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2020

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