Malaria continues to be a global health issue, with almost half of the globe's population living in areas at risk of infection. Globally, a reduction in the number of deaths caused by malaria was recorded between 2000–2019. However, due to the COVID-19 pandemic, continual Ebola outbreaks, and ongoing humanitarian crises, 2020 saw a 12% increase in the number of malaria deaths. Thus, the World Health Organization has called for reinvigorated action to combat malaria. In an effort to contribute to this goal, this Methods Collection aims to create a comprehensive catalogue of videos showcasing methodologies and techniques currently used to study the many complex facets of malaria biology. These include host-parasite interactions, pathogenesis, infection and immunity, and drug resistance. By showcasing the expertise of fellow researchers in this Methods Collection, we hope to increase the potential for new and fruitful collaborations, as well as to aid researchers to continue to develop novel strategies to answer complex questions in malaria biology.