Nowadays, cardiovascular calcification is an irreversible process associated with aging and aging-related pathologies. Moreover, vascular calcification is a silent disease and is diagnosticated when there is no reversible and is usually untreatable. Therefore, diagnostic methods are crucial in the generation, progression, and diagnosis of cardiovascular calcification. Some methodologies have confirmed cardiovascular calcification based on the calcium and phosphate balance levels, proteins involved in bone formation increased, proteins involved in initiating vascular calcification changes, et cetera. Moreover, some techniques determine calcification, such as alizarin red (mainly qualitative staining) and phenolsulphonephthalein dye, to assess quantitative calcium deposition. 

This collection brings together the range of techniques developed to study cardiovascular calcification generation, progression, and diagnosis in vitro (culture cells) and in vivo (animal models and humans). We also aim to include contemporary techniques for vascular calcification evolution and detection. Thus, knowing the old fashion and state-of-the-art methodologies that lead to and improve the ability to detect the generation and progression of cardiovascular calcification will enable a better understanding of aging and age-related diseases allowing for early intervention.