Guest Editors
Jason Miller
University of Michigan, Kellogg Eye Center
Jason Miller, MD, PhD, is the James Grosfeld Endowed Professor and Assistant Professor in the Department of Ophthalmology, Kellogg Eye Center, at the University of Michigan. He completed his MD and PhD at University of California, San Francisco (UCSF) and the Gladstone Institute of Neurological Disease under the mentorship of Steve Finkbeiner, MD, PhD, and did residency and fellowship training at the University of Michigan. He currently directs the James Grosfeld Initiative in Dry Age-Related Macular Degeneration, a comprehensive translational research program aimed at dry AMD. As a physician-scientist, his clinical practice is geared towards patients with AMD, and his bench science lab is dedicated to RPE cell biology relevant for dry AMD, with a particular emphasis on RPE lipid handling.
Qitao Zhang
University of Michigan, Kellogg Eye Center
Qitao Zhang, PhD, is a senior research fellow in the Department of Ophthalmology, Kellogg Eye Center, at the University of Michigan. He obtained his PhD from Peking University in China. Qitao is an RPE biologist with extensive experience utilizing primary and induced pluripotent stem cell-derived RPE cultures to probe pathways relevant for dry AMD.
Collection Overview
Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the developed world. Short of treating pathologic neovascularization that sometimes arises as the disease progresses, there are no approved therapies for the ongoing degeneration. AMD likely involves some combination of retinal pigment epithelial (RPE) dysfunction, abnormal Bruch’s membrane composition, and choriocapillaris atrophy. This collection aims to compile recent methodologic advances in the field of dry AMD bench science that will improve translational discovery for this disease. This collection will focus on a range of “hot topics” of broad interest to the dry AMD research community, including methods for autophagy measurement in the retina and RPE, induced pluripotent stem cell-derived RPE and choroid differentiation, AI-based assessment of RPE morphology, modeling RPE hypoxia in vitro, quantifying Bruch’s membrane thickness and deposits accurately, and other topics.
Articles
Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration
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Cited by 10
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2023
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Jeffrey D. Messinger1, Max Brinkmann2, James A. Kimble1, Andreas Berlin1, K. Bailey Freund3,4, Gregory H. Grossman5, Thomas Ach*6, Christine A. Curcio*1
1Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, 2Department of Ophthalmology, University Hospital of Zurich, 3Vitreous Retina Macula Consultants of New York, 4Department of Ophthalmology, New York University Grossman School of Medicine, 5Advancing Sight Network, 6Department of Ophthalmology, University Hospital Bonn
Maintaining and Assessing Various Tissue and Cell Types of the Eye Using a Novel Pumpless Fluidics System
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Cited by 2
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2023
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LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
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Cited by 5
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2023
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Long-term Monitoring of Oxygen Consumption Rates in Highly Differentiated and Polarized Retinal Pigment Epithelial Cultures
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Cited by 1
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2024
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Qitao Zhang*1, Daisy Y. Shu*2, Richard A. Bryan3, John Y. S. Han1, Gillian A. Gulette1, Kin Lo3, Leo A. Kim4, Jason M. L. Miller1,5
1Kellogg Eye Center, University of Michigan, Ann Arbor, 2School of Optometry and Vision Science, University of New South Wales, 3Lucid Scientific, 4Schepens Eye Research Institute of Mass. Eye and Ear, Department of Ophthalmology, Harvard Medical School, 5Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
Abstracts
AMD in a dish– a novel approach for high-throughput evaluation of drusen deposit under retinal pigment epithelium
Hong Sun1,
Vilas Wagh*1,
Qingming Hou1,
Janmeet Saini1,
Sharad Mittal1,
Rafael Fernandez1,
Vanessa Peterson1,
Kanagaraj Subramanian1
1Genome and Biomarker Sciences, Merck & Co., Inc., Cambridge, MA, USA
Efficient Generation of Retinal Pigment Epithelium from Induced Pluripotent Stem Cells
Devika Bose1,
Kapil Bharti*1,
Ruchi Sharma*1,
Davide Ortolan1,
Jair Montford1
1NEI/NIH
High-throughput workflow for reliable imaging of retinal photoreceptor phagosome populations
Lara A. Skelton1,
Steven J. Fliesler1,
Sriganesh Ramachandra Rao*2
1Research Service, VA Western NY Healthcare System, Buffalo, NY, United States,
2State University of New York, University at Buffalo
Improved Lipofuscin Accumulation Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Differentiated and Polarized Human Retinal Pigment Epithelial Cultures
Qitao Zhang1,
Jason Miller*1,
Gillian Autterson1
1Kellogg Eye Center, University of Michigan
Advanced live imaging of the retinal pigment epithelium to identify causal mechanisms of age-related macular degeneration
Li Xuan Tan1,
Colin Germer2,
Nilsa La Cunza2,
Sydney Williams2,
Aparna Lakkaraju*2,
Renuka Chaphalkar1
1Department of Ophthalmology, University of California, San Francisco,
2Pharmaceutical Sciences and Pharmacogenomics Graduate Program, Department of Ophthalmology, University of California, San Francisco
Characterization and quantification of lipid deposits in mono and polygenic retinal degenerations using iPSC-derived retinal pigment epithelium
Jiwon Ryu1,
Zander Esh1,
Devika Bose1,
Kapil Bharti*1,
Ruchi Sharma1,
Andrei Volkov1,
Sharanya Suresh1,
Mitra Farnoodian1
1North Bethesda, Maryland
Procedures for the Use of the BaroFuse to Maintain and Assess Isolated Retina, Eyecups, RPE Cells and Cell Lines.
Ian R. Sweet*1,
Matthew Grumbine2,
Khang Bao2,
Trevor Crup Crupi2,
Kedar Mokate1,
Gamal Khalil2,
Daniel Cook2,
Rayne Lim3,
Jennifer Chao3,
Brian M. Robbings4,
Daniel Hass4,
James B. Hurley4
1University of Washington Medicine Diabetes Institute, University of Washington, Seattle, WA, 98109 ,
2EnTox Sciences, Inc, Seattle, WA 98195 ,
3Department of Ophthalmology, University of Washington, Seattle, WA 98195 ,
4Department of Biochemistry, University of Washington, Seattle, WA, 98109