Obesity, type-2 diabetes, and non-alcoholic fatty liver disease (NAFLD) have become an epidemic in the 21st century. In addition to increasing the risk of early death due to a vast array of cardiovascular or liver problems, metabolic disorders have been associated with neurological conditions, including Alzheimer’s and Parkinson’s disease. Chronic inflammation, insulin resistance, dysbiosis, and cholestasis have all been suggested as potential factors linking metabolic and brain diseases. Moreover, high sugar and high-fat diets can also induce various functional and structural changes in the brain besides their role in the pathogenesis of NAFLD or type-2 diabetes. 

This collection presents articles on the novel in vitro and in vivo methods and translational models to investigate the connection between metabolic and brain disease. Specifically, it will function as a group of protocols that will help to disseminate innovative approaches to study the gut-liver-brain axis and its role in neurodegeneration. In addition, the collection welcomes cutting-edge techniques that may require refining and testing to improve the reproducibility and consistency of methods to study the effect of dietary interventions on brain health.