Injuries to the nervous system are one of the most challenging problems due to their intrinsically low regeneration capacity. Nerve tissue engineering strategies include the use of nerve conduits, biomaterial scaffolds, decellularized matrices, 3D bio-printed substrates, etc. In recent years, increased progress is being made on decellularized scaffolds, which are shown to provide a promising strategy for nerve regeneration. Decellularized extracellular matrix (dECM) is gaining popularity in scaffold engineering as it preserves the native 3D architecture and proteins of the ECM. Thereby, the use of decellularized matrices has garnered enormous attention from researchers as a scaffold material as well as bio-ink for 3D bioprinting. After successful decellularization, a process called recellularization is employed. It involves the repopulation of acellular ECM scaffolds to generate various types of engineered constructs with specific structures and functions. However, the process involves certain intricate details to be followed in order to be a successful strategy. Therefore, the objective of this collection is to share standard protocols or novel methods for the use of cell or tissue-derived ECM as a biomimetic scaffold material specifically for the development of nerve guide conduits or scaffolds for nerve regeneration. This collection will bring out various design strategies used for developing such promising materials for nerve tissue engineering.