Advances in surgical techniques, immunosuppressive drugs, and intensive care medicine allowed organ transplantation to be standard therapy for end-stage organ disease. Successful organ transplantation can improve patient survival and quality of life. Nonetheless, research is performed constantly to solve challenges in this sector. While normothermic machine perfusion has received much attention recently, cold stress-induced cellular injury is a major clinical problem as cost-efficient static cold storage is the gold standard for organ preservation. Despite the reduced incidence of acute rejection and improved short-term clinical outcomes, current therapies largely fail to benefit long-term graft survival due to ensuing chronic rejection. Thus, translational research using animal models is essential to overcome the current difficulties in organ transplantation.

The purpose of this Methods Collection is to highlight research procedures/methods in the field of transplantation. Authors are welcome to submit manuscripts on, but not limited to, the following topics:

• Animal transplant models (liver, intestine, heart, lung, kidney)
• Vascularized composite allografts
• Cell isolation methods
• Extracorporeal/machine perfusion
• Tissue engineering in transplantation
• Regenerative medicine in transplantation