Over the past decade, there has been rapid growth in the understanding of cancer stem cells (CSC). CSCs exhibit characteristics similar to those of tissue stem cells, such as self-renewal and asymmetric division, which generate daughter cells intended for differentiation and tissue regeneration. Initially, the focus of CSC research was on identifying and validating markers specific to this subset of cancer cells.

However, current investigations have shifted towards comprehending the biology of CSCs, their role in sustaining tumor growth in different malignancies, and the mechanisms behind their ability to repeatedly generate tumors. The niche cells and extracellular matrix that support self-renewal and survival play a crucial role in regulating stem cell biology. As we gain more knowledge about the pathways essential for CSC features, including signals from the niche, we aspire to effectively target this cell population. The identification of CSCs is closely associated with the cell of origin, which refers to the cells capable of initiating tumor formation when modified. While these cells may share certain characteristics with CSCs, it remains unclear whether they are identical in most instances.

Addressing the key aspects of cancer research and finding answers to these questions would be a pivotal step toward advancing cancer treatments. The main objective of this Methods Collection is to uncover the complete attributes of cancer stem cells and the cells from which cancers originate. This knowledge will serve as a fundamental tool for the development of remarkably effective therapies that cause minimal harm to individuals.