GPCRs, the largest receptor family, play a crucial role in nearly every biological process and exhibit diverse ligands. Almost half of these receptors in humans are olfactory, while the other half serve as targets for over 35% of all marketed drugs. Medically speaking, GPCRs are the most crucial protein family. They typically consist of 7 hydrophobic transmembrane domains and are predominantly located on the plasma membrane. This receptor family exhibits a variety of secondary effectors for signal transduction, allowing for signaling bias. Allosteric modulators have emerged as an interesting strategy for therapeutic development, leveraging this feature. Moreover, GPCRs are present in various intracellular membranous organelles, including the nucleus, endoplasmic reticulum, Golgi apparatus, and mitochondria, enabling location-biased signaling. Studying GPCR biology presents unique challenges, difficulties, and caveats that require specific tools. This collection aims to standardize protocols, reduce ambiguity in analyzing methods, and enhance their interpretations in the field of GPCR research. Additionally, we strive to introduce and expand various methods for investigating different aspects of GPCR biology and signaling. Visual observation of proper protocol execution and analysis is valuable for promoting good laboratory practices and advancing the field, whether focusing on structural studies or biochemical signaling pathways. A comprehensive collection of standardized methods in GPCR biology would greatly advance the field.