A comprehensive investigation of trigeminal neuralgia necessitates innovative methodologies to uncover its intricate pathophysiology and formulate more efficient treatments. This Methods Collection introduces state-of-the-art approaches, encompassing in vivo, in vitro, and clinical methodologies. These approaches aim to enhance the evaluation of TN patients and provide more translational experimental models for research. Included are pioneering translational animal models, assessment of trigeminal pain in vivo, in-depth in vitro analysis, and clinical evaluation of individuals with trigeminal neuralgia. 

These animal models, designed to mirror patient symptoms, offer invaluable insights into disease mechanisms, thereby promoting more decisive treatment strategies. The carefully curated collection bridges the gap between preclinical and clinical domains, fostering significant advancements in trigeminal neuralgia research. By integrating advanced techniques, this compilation paves the way for groundbreaking discoveries in understanding and addressing trigeminal neuralgia.