

Ingrid Burvenich
National Imaging Facility, Australia
Dr. Burvenich received her master's in bioengineering from the University of Ghent (Belgium) in 2001. She earned her PhD in radiopharmaceutical sciences from the University of Ghent in 2007, where she characterized monoclonal antibody 14C5 for molecular detection and therapy of αvβ5-expressing cancers. Subsequently, she joined the Tumor Targeting Laboratory of Prof. Andrew Scott at the Olivia Newton-John Cancer Research Institute (Melbourne, Australia) as a postdoctoral researcher.As a senior research scientist, Dr. Burvenich possesses extensive experience in preclinical molecular imaging and biology, as well as in animal cancer models, targeted therapeutics, and cancer biology. She has played a pivotal role in the preclinical development of multiple bioimaging therapeutic antibodies, facilitating their translation into Phase I bioimaging studies. Since July 2021, she has held the position of National Imaging Facility Fellow for preclinical imaging utilizing PET/MRI 3T and SPECT/CT at the La Trobe-ONJCRI NIF node (Ingrid Burvenich, National Imaging Facility, anif.org.au).

Yaser Hadi Gholami
National Imaging Facility, Australia
Dr. Yaser Hadi Gholami is a physicist specializing in nuclear medicine and conducts extensive research in nanomedicine, radionuclide therapy and imaging, radiation physics and biology, nuclear chemistry, and Monte Carlo simulation. In 2019, he developed a groundbreaking theory for positron annihilation localization using nanoscale magnetization during his time as a visiting research associate at Harvard University and Massachusetts General Hospital (MGH).In 2020, he was honored with the prestigious 2020 Physics Grand Challenges award for his work on a quantum-medicine platform to study the quantum properties of cancer cells through positronium lifetime spectroscopy. Dr. Gholami's collaborations with Harvard Medical School and MGH have led to a novel chelate-free nanoparticle radiolabelling technique for PET/MR imaging and radionuclide therapy.He has collaborated with OncoSil Medical Company to develop personalized 32P microparticle therapy and contributed to achieving IDE approval. Additionally, in partnership with Varian, he has established a radiobiological modeling platform to gain valuable insights into temporal changes in tumor FDG distribution and tumor cell radiosensitivity.

Heidi Espedal
University of Western Australia
Heidi Espedal, PhD, is a research fellow at the University of Western Australia and a facility fellow at the National Imaging Facility (NIF). She specializes in PET-CT and MR imaging. Espedal was the head of the preclinical PET-CT facility at the Molecular Imaging Center at the University of Bergen in Norway for seven years. During her postdoctoral research, she focused on gynecological cancer imaging (PET-CT and MRI) in mouse models, and her doctoral research included PET and MR imaging projects related to orthotopic brain tumors and brain metastases in rodent models. In addition to cancer imaging, Espedal has collaborated on numerous projects involving physiology, tissue engineering, and petroleum imaging. Her primary research interests revolve around dynamic PET imaging, translational imaging, and the development of improved quantification methods.
Positron emission tomography (PET) is a medical imaging technique that involves the utilization of radiopharmaceuticals, which are molecules linked to radioactive substances emitting positrons. Upon injection into an animal or patient, this non-invasive whole-body imaging method offers valuable insights into the in vivo characteristics of tissues at the molecular level in real-time.
PET is a multidisciplinary approach dependent on various elements, including the availability of radioisotopes, probe development, the establishment of relevant animal models, advancements in imaging reconstruction algorithms, and PET instrumentation. After image reconstruction, software-based quantitative analysis calculates the level of radioactivity in each organ of interest. PET has demonstrated its efficacy as a powerful instrument in various domains, including drug discovery, patient diagnosis, personalized medicine, and toxicity prediction.
This Methods Collection aims to highlight the latest techniques in PET imaging research and the diverse preclinical imaging instruments available in the field. The collection encompasses a wide range of topics, including but not limited to:
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2024
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Laurence Morandeau1, Joseph A. Ioppolo1,2, Eva Alvarez de Eulate1, Shifaza Mohamed1, Danica Cullen1, Ali H. Asad1, Roslyn J. Francis2,3,4, Janette Atkinson1
1Radiopharmaceutical Production and Development Centre (RAPID), Medical Technology and Physics Department, Sir Charles Gairdner Hospital, 2National Imaging Facility, Centre for Microscopy, Characterisation and Analysis, University of Western Australia, 3Department of Nuclear Medicine, Sir Charles Gairdner Hospital, 4Medical School, University of Western Australia
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2024
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Betül Altunay1, Matthias Bauwens1,2,3, Laura Schäfer1, Amelie Heesch1, Agnieszka Morgenroth1, Felix M. Mottaghy1,2,4, Alexandru Florea1,2,4
1Department of Nuclear Medicine, University Hospital RWTH Aachen, 2Department of Radiology and Nuclear Medicine, Maastricht University Medical Center, 3School of Nutrition and Translational Research in Metabolism (NUTRIM), Maastricht University, 4School for Cardiovascular Diseases (CARIM), Maastricht University
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2025
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Automated synthesis of [68Ga]Ga-FAPI-46 on the iPHASE MultiSyn Synthesizer
Laurence Morandeau*1,
Joseph Ioppolo2,
Eva Alvarez de Eulate1,
Shifaza Mohamed1,
Danica Cullen1,
Ali Asad1,
Roslyn Francis2,
Janette Atkinson1
1Radiopharmaceutical Production and Development Centre (RAPID), Medical Technology and Physics Department, Sir Charles Gairdner Hospital, Hospital Avenue, Nedlands, Western Australia, Australia,
2National Imaging Facility, Centre for Microscopy, Characterisation and Analysis, University of Western Australia, Crawley, Western Australia, Australia
First-In-Class Diagnostics Targeting Neuropeptide Y1 Receptor (Y1R): Enabling Molecular Imaging of Metastatic Breast Cancer
Marwa Nadia Rahimi1,
Jessica Van Zuylekom2,
Benjamin Blyth2,
Philip Thompson3,
Mohammad Haskali*1
1The Radiopharmaceutical Research Laboratory, The Peter MacCallum Cancer Centre, Victoria, Australia,
2Models of Cancer Translational Research Centre, The Peter MacCallum Cancer Centre, Victoria, Australia,
3Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), Victoria, Australia
Evaluation of absorbed dose to organs from 18F-FDG PET imaging using EGS5 Monte Carlo simulations
Shlomi Caduri1,
Itzhak Orion*2
1Department of Nuclear Engineering, Ben-Gurion University of the Negev|Schneider Children's Medical Center,
2Department of Nuclear Engineering, Ben-Gurion University of the Negev
Comprehensive workflow for radiotracer synthesis, quality assurance, and precise in vivo injections in small animal PET/SPECT/CT imaging
Alexandru Florea*1
1Department of Nuclear Medicine, University Hospital RWTH Aachen, 52074 Aachen, Germany
Aliquoting commercial cold kits for high-efficiency gallium-68 radiolabeling: A practical protocol using Locametz® and SomaKit TOC®
Cyril Fersing*1
1Nuclear Medicine unit, Radiopharmacy team, Montpellier Cancer Institute (Montpellier, France)