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Collection Overview
T helper cells play a central role in orchestrating adaptive immune responses. Extensive research has identified five primary CD4 T helper cell (Th) subsets: Th1, Th2, Th17, Treg (T regulatory), and Tfh (follicular T helper) cells. Additionally, less common T helper subsets, including "Th3", "Th9", and "Th22" cells, as well as subsets of Tregs, have also been recognized. Their roles in various diseases and immune responses make them a critical focus of research in immunology and medicine. This Methods Collection will spotlight both established and emerging techniques related to T helper cell research. It will offer cutting-edge and reproducible protocols with a focus on:
- Studying the in vivo and in vitro development of T helper cells
- Investigating tissue-resident T helper cells
- Utilizing the CRISPR-Cas9 screening system in T helper cell research
- Exploring methods for studying human T helper cells
Articles
Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
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Cited by 1
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2024
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1Shanghai Immune Therapy Institute, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 2Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, 3Institute of Immunology and Department of Rheumatology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice
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2024
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1Department of Urology, South China Hospital, Medical School, Shenzhen University, 2Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University Medical School, 3Cancer Center, Daping Hospital & Army Medical Center of PLA, Third Military Medical University, 4Guangdong Provincial Key Laboratory of Immune Regulation and Immunotherapy, School of Laboratory Medicine and Biotechnology, Southern Medical University, 5Institute of Immunology, Third Military Medical University, 6Dermatology Hospital, Southern Medical University, 7Institute of Immunological Innovation and Translation, Chongqing Medical University
Efficient Tonsillar T Follicular Helper Cell Processing and Functional Analysis through High-dimensional Flow Cytometry
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2025
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Qin Xu1, Lihong Shi1, Brian L. P. Dizon2,3, Rylee J. Beam2, Pamela Mudd4,5, Pamela L. Schwartzberg1, Kalpana Manthiram1
1Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Rheumatology Fellowship Training Program, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, 4Division of Pediatric Otolaryngology, Children's National Hospital, 5Division of Otolaryngology, Department of Surgery, George Washington University School of Medicine and Health Sciences