Bone and teeth are the primary mineralized tissues in vertebrates, including humans. They are highly organized structures that carry out essential functions in movement, requiring dynamic and balanced tissue remodeling to maintain their biological functions. Failure to do so leads to bone and teeth disorders such as osteoporosis, osteonecrosis, and periodontal diseases. Bone and teeth pose challenges for study due to their natural composition of high mineral content. The research methodology for bone and teeth can vary among different labs. 

This Methods Collection aims to gather gold-standard and cutting-edge techniques in current mineralized tissue research, focusing on animal models, tissue or cell analytic techniques, and in vitro cell culture models. We hope this Methods Collection can serve as a valuable reference to further promote basic and translational research in the mineralized tissue field.