Antioxidants are substances that neutralize reactive oxygen species and volatile molecules that cause cellular oxidative stress. Cellular oxidative stress occurs when key biological molecules undergo oxidation. Excessive cellular oxidative stress has been linked to increased susceptibility to cardiovascular and chronic neurodegenerative diseases in humans. Antioxidants, such as polyphenols, carotenoids, terpenes, vitamins, and minerals, occur naturally in plants and animals. Due to their health benefits, antioxidants are used to formulate functional foods and pharmaceuticals. 

Despite the pivotal role of antioxidants, the methodologies employed for their extraction and characterization pose significant challenges and limitations. Traditional approaches often entail labor-intensive processes, exhibit environmental drawbacks, and necessitate exhaustive sample preparation steps. Addressing these challenges mandates exploring novel, efficient, and eco-friendly methods for antioxidant characterization in natural products. 

This Methods Collection aims to highlight diverse methodologies for extracting and characterizing natural antioxidants. It seeks to equip researchers with versatile tools to navigate the complexities of antioxidant analysis by showcasing established techniques, cutting-edge protocols, and innovative practices. Overall, this collection will serve as a comprehensive resource for fostering precision and confidence in research endeavors and promoting collaboration, innovation, and advancement in the field of antioxidant research.