Rheology and mechanics are fundamental aspects of soft matter, which have large response functions. During the past two decades, rheometers, AFM, nanoindenters, and other types of advanced instrumentation have become commercially available. These instruments enable better understanding of the dynamics and structure-processing relationship of soft matter. Yet there are still challenges that exist, such as measuring scarce amounts of biomacromolecules, and in-situ and hyphenated measurements which are not readily accessible in commercial instruments. In this collection, we hope to advocate novel methods that tackle these problems, including, but not limited to: rheometry combined with additional techniques; novel rheological and mechanical measurements; microfluidic devices.