Cardiovascular diseases are the leading cause of death worldwide. Rodent models, tailored to target different vascular beds, have been extensively employed to enhance our understanding of disease development. Coupled with intravital microscopy, it is possible to monitor thrombus formation and development in arterial and venous thrombosis models, as well as endothelial function, not only in vitro but also in vivo. Atherosclerotic genetic rodent models shed light on plaque development and rupture. To mimic pathophysiology and disease progression, researchers apply stenosis, trauma, ischemia-reperfusion injury, or vasorelaxation. Fluorescent dyes and fluorescently labeled antibodies targeting leukocytes or platelets are commonly used to visualize key cells and components mediating thrombus formation at sites such as the carotid and femoral arteries, the vena cava, or even smaller vessels in the mesenteric venules or the cremaster arterioles in real-time. The utilization of these tools enables the discovery of critical factors involved in thrombosis and facilitates the pre-clinical development of new therapeutic approaches. This collection brings together various techniques and tools for the study of cardiovascular diseases.